中国现代中药2025，Vol.27Issue(11)：2025-2030,6.DOI:10.13313/j.issn.1673-4890.20250407005
三线定量胶体金技术快速检测制远志中黄曲霉毒素的不确定度评估
Uncertainty Evaluation of Rapid Detecrmination of Aflatoxins in Polygalae Radix by Three-line Quantitative Colloidal Gold Technology
摘要
Abstract
Objective:To evaluate the uncertainty of the three-line quantitative colloidal gold technology for determining the content of aflatoxins in Chinese medicinal materials and quantify the accuracy and reliability of the rapid detection method.Methods:Aflatoxins in processed Polygalae Radix were quantified using a colloidal gold immunochromatographic assay coupled with a reading device.A mathematical model was established to analyze the sources of measurement uncertainty.Results:The measurement repeatability,standard curve fitting,sample extraction and dilution,and weighing process all brought uncertainty.Standard curve fitting(relative uncertainty of 0.088 1)was the main factor among them.The combined relative standard uncertainty was 0.092 3,and the expanded uncertainty was 1.363(k=2).Conclusion:The three-line quantitative colloidal gold technology could satisfy the determination requirements of aflatoxins.However,it is essential to optimize the standard curve fitting algorithm and enhance temperature control.关键词
胶体金免疫层析法/黄曲霉毒素/不确定度/快速检测/定量测定Key words
colloidal gold immunochromatographic assay/aflatoxin/uncertainty/rapid detection/quantitative determination分类
医药卫生引用本文复制引用
张文婷,傅欣彤,李铮,王萌萌,朱志晓,刘龙飞,管阳..三线定量胶体金技术快速检测制远志中黄曲霉毒素的不确定度评估[J].中国现代中药,2025,27(11):2025-2030,6.基金项目
国家重点研发计划项目(2023YFC3504103) （2023YFC3504103）
