中国现代中药2025，Vol.27Issue(11)：2047-2060,14.DOI:10.13313/j.issn.1673-4890.20250423002
基于DI-MS/MSALL的山楂化学成分快速解析
Rapid Analysis of Chemical Constituents of Crataegi Fructus Based on DI-MS/MSALL
摘要
Abstract
Objective:To rapidly characterize the chemical constituents of Crataegi Fructus using direct infusion-tandem mass spectrometry with full scanning(DI-MS/MSALL).Methods:The extract of Crataegi Fructus was directly introduced into a quadrupole time-of-flight mass spectrometer(Q-TOF-MS)via the chromatogram-free direct infusion mode at a flow rate of 10 μL·min-1.Full-scan mass data were acquired in both positive and negative ion modes using MS/MSALL.An MS1-MS2 data list was constructed,and compounds were identified based on mass fragmentation patterns and relevant literature.Results:A total of 44 compounds were tentatively identified from the extract,including 15 organic acids,13 flavonoids,5 triterpenoids,9 amino acids,and 2 sugars.Conclusion:The DI-MS/MSALL enables rapid qualitative analysis of the chemical constituents in Crataegi Fructus,providing a valuable dataset for its further development and utilization.This technique integrates the high throughput advantages of DI-MS with the high resolution and strong specificity of Q-TOF-MS,offering an efficient tool for rapid compositional profiling of complex systems such as traditional Chinese medicines.关键词
直接注射-多级质谱全扫描法/山楂/化学成分/质谱裂解途径Key words
DI-MS/MSALL/Crataegi Fructus/chemical constituents/mass spectrometry fragmentation pathways分类
医药卫生引用本文复制引用
曹耘畅,张梦瑶,白润梓,张珂,宋月林,谢彩侠,刘文静..基于DI-MS/MSALL的山楂化学成分快速解析[J].中国现代中药,2025,27(11):2047-2060,14.基金项目
河南省自然科学基金项目(252300421619) （252300421619）
重大疾病新药靶发现及新药创制全国重点实验室开放课题(SKLD2024Q01) （SKLD2024Q01）
