中国现代中药2025，Vol.27Issue(11)：2061-2072,中插4,13.DOI:10.13313/j.issn.1673-4890.20241129002
LC-MS非靶向代谢组学结合HPLC分析不同加工温度对玄参化学成分的影响
LC-MS Non-targeted Metabolomics Combined with HPLC for Analysis of Effects of Different Processing Temperature on Chemical Components of Processed Scrophulariae Radix
摘要
Abstract
Objectives:To explore the effects of different processing temperatures on the chemical components of Scrophulariae Radix root tuber and identify the optimal processing temperature using traditional processing as a reference.Methods:Adjacent tissue of root tubers from the same Scrophulariae Radix were used as study material.Freeze-drying,low-temperature drying,high-temperature drying,and traditional drying treatments were performed.Non-targeted metabolomics based on liquid chromatography-mass spectrometry(LC-MS)was applied to comprehensively detect and analyze the differences in compound profiles among the treatments.High performance liquid chromatography(HPLC)was applied to quantify representative cyclic ether terpenoids and phenylpropanoids in each treatment.Results:Non-targeted metabolomics results indicate that metabolites profiles of the low-temperature(60℃)drying group and the traditional processing group cluster together,with the fewest differential metabolites between the two groups.Both the high-temperature drying group and the freeze-drying group were clearly separated from other processing groups.Compared with the traditional processing group,347 metabolites in the 80℃drying group were up-regulated,far exceeding the number in the 60 ℃ drying group.In contrast,the freeze-drying group had 209 down-regulated differential metabolites,with both the number and proportion higher than those in the drying and traditional processing groups.Kyoto Encyclopedia of Genes and Genomes(KEGG)annotation showed higher enrichment factors in D-arginine and D-ornithine metabolism,glycerophospholipid metabolism,and other metabolic pathways.HPLC analysis indicates that the contents of pharmacodynamic components in the low-temperature(60℃)drying group and the traditional processing group clustered together,whereas the high-temperature(70,80,and 90℃)drying groups and the freeze-drying group were significantly separated from other processing groups.The contents of aucubin and angoroside C decrease gradually as the processing temperature increases.Cinnamic acid content was the lowest in the freeze-drying group and increases significantly with higher processing temperatures.The contents of harpagide and verbascoside first increase and then decrease as processing temperature rises.The variation trend of harpagoside content is completely opposite to that of harpagide.In the 60℃drying group,harpagide reaches 0.94%,showing little difference from the traditional processing group but significantly higher than other treatments.Conclusion:Drying at 60℃preserves pharmacologically active components in processed Scrophulariae Radix pieces similarly to the traditional low-temperature-sweat-low-temperature drying.关键词
玄参/温度/液相色谱-质谱法/高效液相色谱法/差异代谢物Key words
Scrophulariae Radix/temperature/LC-MS/HPLC/differential metabolite分类
医药卫生引用本文复制引用
蔡龙,姚晖,孙健,王盼,邱萍,周潮滨,王志安..LC-MS非靶向代谢组学结合HPLC分析不同加工温度对玄参化学成分的影响[J].中国现代中药,2025,27(11):2061-2072,中插4,13.基金项目
中央本级重大增减支项目(2060302-2202-08) （2060302-2202-08）
磐安县中药产业"揭榜挂帅"项目(PZYF202101) （PZYF202101）
浙江省农业新品种选育重大科技专项(2021C02074) （2021C02074）
国家中医药管理局中医药科学技术研究专项(GZY-KJS-2021-020) （GZY-KJS-2021-020）
浙江省中医药科技计划中医药现代化专项(2022ZX003) （2022ZX003）
