中国现代中药2025，Vol.27Issue(11)：2073-2082,10.DOI:10.13313/j.issn.1673-4890.20250507002
基于指纹图谱与一测多评法的照山白质量评价方法研究
Study on Quality Evaluation Method of Rhododendron micranthum Based on Fingerprint and Quantitative Analysis of Multi-components by Single-marker
摘要
Abstract
Objective:To establish a fingerprint and a quantitative analysis of multi-components by single marker(QAMS)for the simultaneous determination of six components in Mongolian medicine Rhododendron micranthum and evaluate its quality.Method:An Agilent Eclipse Plus C18 column was used with a mobile phase consisting of 0.2%phosphoric acid solution(A)-acetonitrile(B)-tetrahydrofuran(C)with gradient elution(0-70 min,5%-20%B,2%-7%C)at a detection wavelength of 290 nm,so as to establish the high performance liquid chromatography(HPLC)fingerprints of seven batches of Rhododendron micranthum.Hyperoside was selected as the internal reference substance to develop the QAMS method for quantifying the other five components,and their contents were calculated using relative correction factors.The results were compared with those obtained by the external standard method(ESM)to evaluate the accuracy of QAMS.Results:The HPLC fingerprints revealed 20 common peaks in the seven batches of Rhododendron micranthum,with gallic acid,chlorogenic acid,hyperoside,isoquercitrin,avicularin,and quercitrin being assigned.The similarity between the sample fingerprints and the reference fingerprint ranged from 0.906 to 0.992.The relative standard deviation(RSD)of the above six components measured by QAMS and ESM was less than 3.0%.The content ranges of the six components were 0.083-0.121,0.041-0.206,2.422-7.844,1.934-4.234,0.334-2.477,and 3.205-6.424 mg·g-1,respectively.Conclusion:The HPLC fingerprint combined with the QAMS method is simple,accurate,and reproducible,providing a reliable approach for improving the quality control standards of Rhododendron micranthum.关键词
蒙药材/照山白/指纹图谱/一测多评/质量评价Key words
Mongolian medicine/Rhododendron micranthum Turcz./fingerprint/quantitative analysis of multi-components by single marker/quality evaluation分类
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孟和,赵雪莲,玉华,红艳,杨立国,嵇增云,郝俊生,辛颖..基于指纹图谱与一测多评法的照山白质量评价方法研究[J].中国现代中药,2025,27(11):2073-2082,10.基金项目
2024年蒙药研发国家地方联合工程研究中心开放课题(MDK2024009) （MDK2024009）
国家药品监督管理局中药质量研究与评价重点实验室开放课题(NIFDC-TCM2025-0201-030) （NIFDC-TCM2025-0201-030）
