草原与草坪2025，Vol.45Issue(5)：13-25,13.DOI:10.13817/j.cnki.cyycp.2025.05.002
高寒矿区人工恢复草地与自然植被土壤酶活性与土壤微生物特征比较分析
Comparative analysis of soil enzyme activity and soil microbial characteristics between artificially restored grassland and natural vegetation in high-altitude mining areas
摘要
Abstract
[Objective]To elucidate the restoration effects and key influencing factors of soil enzyme activity and soil microbial community structure during ecological restoration in high-altitude mining areas.[Method]A com-parative study was conducted to examine the activity of four soil enzymes involved inC,N,and P cycles,namely β-glucosidase(βG),leucine aminopeptidase(LAP),N-acetyl-β-D-glucosidase(NAG),and acid phosphatase(ACP),as well as soil microbial community diversity,and composition.Samples werecollected from artificial grass-land(RS:reconstructed soil artificial grassland;NRS:unreformed soil artificial grassland)and natural vegetation(AM:alpine meadow;ASM:alpine swamp meadow)soils.[Result]The activities of the four soil enzymes showed similar patterns across soil layers.The highest LAP was observed in ASM treatment,followed by RS,with no signifi-cant difference between the two treatments(P＞0.05).The activities of the other three soil enzymes were higher in artificial grassland treatment than in native grassland,with the βG activity significantly higher in artificial grassland than in native grassland(P＜0.05).Microbial community diversity showed that there was a significant difference in soil bacterial diversity between artificial grassland and native grassland(P＜0.05).The structure and composition of bacterial communities changedmarkedly,with increasedrelative abundance of Proteobacteria and Actinobacteria and decreasedabundance of Acidobacteria and Chloroflexia inartificial grasslands compared to native natural grasslands.has The content of TC and TP in RS treatment was significantly higher than that in AM and ASM treatment(P＜0.05);The content of TN,NO3-N,and AP all showed a trend of ASM＞AM＞RS＞NRS;The soil respiration rate RS was significantly higher than the other three treatments(P＜0.05),while the net ecosystem exchange rate RS and ASM were significantly lower than AM and NRS(P＜0.05).[Conclusion]Correlation analysis results indi-cated that artificial grassland establishment can progressively enhance soil enzyme activity,microbial community di-versity,and microbial community structure by improvingsoil microenvironment factors such as temperature,humidity,pH,C,N,P element content,vegetation diversity.These improvements contribute to the restoration of degraded al-pine mining grasslands and promote long-term ecosystem stability.关键词
高寒矿区/人工草地/土壤酶活性/微生物/群落多样性/群落结构/环境因子Key words
high altitude mining area/artificial grassland/soil enzyme activity/microbial/community diversity/community structure/environmental factor分类
农业科技引用本文复制引用
HUO Ya-nan,ZHANG Shi-bin,YANG Yuan-wu,ZHENG Jie,XIAO Yuanyuan..高寒矿区人工恢复草地与自然植被土壤酶活性与土壤微生物特征比较分析[J].草原与草坪,2025,45(5):13-25,13.基金项目
中央林业改革发展资金天峻县木里矿区种草复绿区合理利用试验研究项目(E6301000076043631001001) （E6301000076043631001001）
中央财政林草科技推广示范项目(青[2021]TG01号) （青[2021]TG01号）
青海省科技创新创业团队项目 （）
高等学校学科创新引智计划项目(D18013) （D18013）
