草原与草坪2025，Vol.45Issue(5)：66-75,10.DOI:10.13817/j.cnki.cyycp.2025.05.007
外源NO对酸胁迫下夏枯草种子萌发和幼苗生理的影响
Effects of exogenous nitric oxide on seed germination and seedling growth of Prunella vulgaris under acid stress
摘要
Abstract
[Objective]This study aimed to investigate the effects of exogenous nitric oxide(NO)on seed germi-nation and seedling physiology of Prunella vulgaris under acid stress.[Method]Seeds of P.vulgaris were soaked in sodium nitroprusside(SNP)solutions at different concentrations(0.01,0.05,0.1,0.5 and 1 mmol/L),using SNP solution as an exogenous nitric oxide donor.The seeds were then subjected to acid stress(pH=3.0).Germination parameters and seedling physiological indices were measured to evaluate the mitigating effects of exogenous NO un-der acid stress.[Results]Acid stress significantly inhibited seed germination,decreased photosynthetic pigment con-tent,and increased relative conductivity in P.vulgaris.In contrast,different concentrations of SNP effectively allevi-ated the acid-induced damage.The mitigation effect increased initiallyand then declinedwith rising SNP concentra-tion,with 0.1 mmol/L SNP showing the most pronounced improvements.Compared with the acid-stress control,the germination rate,germination potential,germination index,and vigor index increased by 36.36％,45.30％,46.32％and 75.83％respectively,while the germination recovery rate reached 28.57％.Root length,seedling height,fresh weight,and total chlorophyll content increased by 59.42％,23.21％,19.12％,and 19.27％,respec-tively,and relative conductivity decreased by 28.78％.Principal component analysis indicated that the effects of exog-enous NO on seed germination ability of P.vulgaris,under acid stress,were as follows:0.1 mmol/L SNP＞0.5 mmol/L SNP＞1 mmol/L SNP＞0.05 mmol/L SNP＞0.01 mmol/L SNP.[Conclusion]An appropriate con-centration of exogenous NO(0.1 mmol/L SNP)significantly enhances seed germination rate and seedling vigor of P.vulgaris under acid stress by reducing membrane damage and promoting photosynthetic chlorophyll accumulation.These findings provide a theoretical basis and technical reference for the ecological restoration and lands capeculiva-tionof P.vulgaris in acidified soils.关键词
夏枯草/外源一氧化氮/酸胁迫/种子萌发/幼苗生理/光合色素Key words
Prunella vulgaris/exogenous nitric oxide/acid stress/seed germination/physiology of seedlings/photosynthetic pigment分类
农业科技引用本文复制引用
CHANG Qing-shan,ZHANG Li-xia,Zhang Qiao-ming,HU Shi-bing,Yang ming-ying,LI Shu-bing,ZHENG Song-lin,HOU Xiao-gai..外源NO对酸胁迫下夏枯草种子萌发和幼苗生理的影响[J].草原与草坪,2025,45(5):66-75,10.基金项目
河南省现代农业产业技术体系建设专项资金项目(豫财科2022-24号) （豫财科2022-24号）
河南省自然科学基金项目(242300420144) （242300420144）
河南省科技攻关项目(242102110158,242102320272) （242102110158,242102320272）
河南省级科技研发计划联合基金项目(222103810057) （222103810057）
大学生创新创业训练计划项目(2023450) （2023450）
