南方医科大学学报2025，Vol.45Issue(12)：2527-2540,14.DOI:10.12122/j.issn.1673-4254.2025.12.01
金银花提取物对阿霉素诱导的小鼠心肌损伤的保护作用及其机制
Protective effect of Lonicerae Japonicae Flos extract against doxorubicin-induced myocardial injury in mice and the possible mechanisms
摘要
Abstract
Objective To evaluate the protective effect of Lonicerae Japonicae Flos(LJF)extract against doxorubicin(DOX)-induced cardiotoxicity(DIC)and explore the possible mechanisms.Methods Network pharmacology,bioinformatics analysis and molecular docking were used to predict the targets of the core components of LJF.In a mouse model of DOX-induced myocardial injury,the protective effects of different doses of LJF extract were evaluated and the underlying mechanisms were explored by detecting the changes in mouse myocardial functions,myocardial enzymes,myocardial pathologies,and the expressions of inflammatory factors and pyroptosis-related proteins.Results The 10 core ingredients of LJF showed strong binding to AKT,EGFR,and GSK3β.In the animal experiment,the DOX-treated mice,compared with the sham-treated mice,had significantly decreased cardiac output,stroke volume,left ventricular ejection fraction,left ventricular fraction shorting,elevated serum levels of CK-MB and LDH,increased myocardial expressions of IL-18 and IL-1β,obvious myocardial damage,increased expression levels of NLRP3,caspase-1,GSDMD and GSDMD-N,and reduced expressions of EGFR,p-AKT and p-GSK3β proteins in the myocardial tissues.LJF treatment obviously improved myocardial function,decreased myocardial expressions of IL-18,IL-1β,NLRP3,caspase-1,GSDMD and GSDMD-N proteins,and increased the expressions EGFR,p-AKT and p-GSK3β proteins in DOX-treated mice.Conclusion LJF extract alleviates DOX-induced myocardial injury in mice possibly by reducing myocardial inflammation and pyroptosis via targeting EGFR,AKT and GSK3β to regulate the ErbB signaling pathway.关键词
网络药理学/金银花提取物/阿霉素/分子对接/细胞焦亡/ErbB信号转导通路Key words
network pharmacology/Lonicerae Japonicae Flos extract/doxorubicin/molecular docking/pyroptosis/ErbB signaling pathway引用本文复制引用
XIA Shicheng,WEI Huifang,HONG Weican,ZHANG Yuming,YIN Feiyang,ZHANG Yixin,ZHANG Linlin,GAO Qin,YE Hongwei..金银花提取物对阿霉素诱导的小鼠心肌损伤的保护作用及其机制[J].南方医科大学学报,2025,45(12):2527-2540,14.基金项目
Supported by Anhui Provincial Funds for Excellent Scientific Research and Innovation Team(2022AH010083),512 Talent Program of Bengbu Medical University(by51201102),and Innovation and Entrepreneurship Training Program for College Students(S202410367081,S202410367011,202410367058,S202410367057). 安徽省高校优秀科研创新团队项目(2022AH010083) （2022AH010083）
蚌埠医科大学"512人才计划"(by51201102) （by51201102）
大学生创新创业训练计划项目(S202410367081,S202410367011,202410367058,S202410367057) （S202410367081,S202410367011,202410367058,S202410367057）
