分析化学2025，Vol.53Issue(11)：1761-1772,12.DOI:10.19756/j.issn.0253-3820.251222
微流控-成簇规律间隔短回文重复序列协同检测技术在临床病原体即时检验中的研究进展
Progress of Microfluidics-Integrated Clustered Regularly Interspaced Short Palindromic Repeats Detection Systems for Pathogen Diagnosis in Point-of-Care Testing
摘要
Abstract
Clustered regularly interspaced short palindromic repeats-associated proteins(CRISPR-Cas)system has emerged as a next-generation nucleic acid detection tool due to its precise gene editing capabilities and exhibits high specificity and significant potential for application in the diagnosis of pathogen.However,the inherent shortcomings of CRISPR-Cas,including insufficient sensitivity of the traditional CRISPR-Cas system,complex operation of the CRISPR-Cas system combined with amplification,and limited capability for multi-target detection,have significantly constrained its practical applications in clinical settings.With the advantages of miniaturization,integration and automation,microfluidics has emerged as a promising tool for advancing the development of point-of-care testing(POCT)platforms.The integration of microfluidic technology with CRISPR-Cas system not only enhances the detection throughput and efficiency significantly,but also drives innovation in the development of portable POCT devices.In this paper,a comprehensive review of the research advancements of CRISPR-Cas nucleic acid detection platform facilitated by microfluidic technology was presented,with a focus on their application in detection of pathogens associated with clinical disease.This study aimed to explore emerging molecular diagnostic methodologies and offered novel insights for future research in clinical pathogen detection.关键词
成簇规律间隔短回文重复序列及其相关蛋白/微流控技术/即时检验/核酸检测/评述Key words
Clustered regularly interspaced short palindromic repeats-associated proteins/Microfluidics/Point-of-care testing/Nucleic acid detection/Review引用本文复制引用
XU Qian,ZHANG Jing,QIN Yu-Ling,WU Li..微流控-成簇规律间隔短回文重复序列协同检测技术在临床病原体即时检验中的研究进展[J].分析化学,2025,53(11):1761-1772,12.基金项目
国家自然科学基金项目(Nos.82373456,32171452)、江苏省杰出青年基金项目(No.BK20220060)和江苏省双创团队项目(No.JSSCTD202348)资助. Supported by the National Natural Science Foundation of China(Nos.82373456,32171452),the Excellent Youth Foundation of Jiangsu Scientific Committee(No.BK20220060)and the Jiangsu Innovative and Entrepreneurial Research Team Program(No.JSSCTD202348). （Nos.82373456,32171452）
