干旱地区农业研究2025，Vol.43Issue(6)：1-11,11.DOI:10.7606/j.issn.1000-7601.2025.06.01
237份小麦穗部性状的全基因组关联分析
Genome-wide association study of spike-related traits in 237 wheat accessions
摘要
Abstract
In this study,237 wheat germplasms(185 ICARDA-introduced wheat accessions and 52 domestic core germplasms)were used as the materials to investigate the four traits of spike length,number of spikes per plant,number of grains per spike,and number of spikelets per spike under eight environmental conditions.The re-sults showed that the four spike-related traits exhibited extensive variation across the eight environments,with the minimum variation range of spike length(9.63%to 13.22%)and the maximum variation range of spike grain num-ber per spike(9.65%to 25.45%).Then,GWAS of four spike-related traits were performed using a multi-locus random SNP effect mixed linear model based on the genotyping data from a 660K SNP array analysis.A total of 506 significant loci associated with spike traits were identified,including 148 for spike length,107 for number of spikes per plant,138 for number of grains per spike,and 113 for number of spikelets per spike.Based on LD and genome annotation information,490 candidate genes associated with spike length were obtained,as well as 164 genes asso-ciated with the number of spikes per plant,181 genes associated with the number of grains per spike,and 81 genes associated with the number of spikelets per spike,of which 37 associated SNPs were found in its genetic regions.Based on haplotype analysis,we ultimately obtained seven main candidate genes,namely TraesCS1A02G285000,TraesCS4D02G029400,TraesCS2D02G155000,TraesCS1D02G383400,TraesCS5B02G274700,TraesCS6A02G002500,and TraesCS2D02G018300.This study provided the material and genetic resources for overcoming genetic homogeniza-tion and breeding high-yield new wheat varieties through utilizing the introduced germplasm.关键词
小麦/全基因组关联分析/穗部性状/候选基因Key words
wheat/genome-wide association study/spike-related traits/candidate genes分类
农业科技引用本文复制引用
HAO Junwei,HE Zhuoheng,LUO Shuang,YANG Wenqi,NIE Xiaojun..237份小麦穗部性状的全基因组关联分析[J].干旱地区农业研究,2025,43(6):1-11,11.基金项目
农业农村部农业生物育种重大专项(2023ZD0402601) （2023ZD0402601）
国家自然科学基金项目(U22A20457) （U22A20457）
