干旱地区农业研究2025，Vol.43Issue(6)：12-22,38,12.DOI:10.7606/j.issn.1000-7601.2025.06.02
陆地棉萌发期和三叶期耐复合盐种质筛选与鉴定
Screening and identification of upland cotton germplasm with tolerance to compound salt stress at germination and three-leaf stages
摘要
Abstract
To screen for cotton genotypes with tolerance to composite salt stress,166 cotton genotypes were used as experimental materials and subjected to compound salt stress at concentrations of 0.15 mol·L-1 and 0.30 mol·L-1.During the germination stage,genotypes were evaluated using comprehensive ranking and fuzzy member-ship function methods to identify those with greater tolerance.The selected genotypes,along with the control varie-ties'Zhong 9807'(salt-tolerant)and'Xinong 606'(salt-sensitive),were grown to the three-leaf stage and sub-jected to compound salt stress.The measurements of leaf morphology,relative chlorophyll content,gas exchange parameters,and chlorophyll fluorescence were conducted to further assess their tolerance.The results showed that seed germination and embryo root growth were significantly inhibited under 0.15 mol·L-1 compound salt-alkali stress in most genotypes.Specifically,the germination vigor,germination rate,germination index,fresh weight,and embryo root length decreased by 72.558%,63.542%,74.737%,52.790%,and 17.080%,respectively,com-pared with the control group treated with distilled water.However,32 genotypes screened using germination traits showed relatively mild inhibition.At 0.30 mol·L-1 concentration,the inhibitory effects were significantly greater than those observed at 0.15 mol·L-1,with five measured traits showing reductions of 96.247%,90.909%,95.389%,98.097%,and 97.602%,respectively.Through comprehensive evaluation,two compound salt-tolerant genotypes,'De 93-04'(ID:X170743)and'4SA-805'(ID:X170523),were ultimately identified.Three-leaf stage evalua-tions confirmed that these two genotypes maintained larger leaf areas and higher photosynthetic activity than the con-trols under high-concentration composite salt stress,indicating their strong tolerance at the seedling stage.关键词
陆地棉/种质/复合盐胁迫/萌发期/三叶期Key words
upland cotton(Gossypium hirsutum L.)/germplasm/compound salt stress/germination stage/three-leaf stage分类
农业科技引用本文复制引用
LUO Xiheng,LEI Zhangying,HE Daohua,ZHANG Tong,WU Yanqing,WANG Peiyan,TIAN Ziyi,TANG Qing,LIU Jiao,YANG Yanlong,HAN Huanyong..陆地棉萌发期和三叶期耐复合盐种质筛选与鉴定[J].干旱地区农业研究,2025,43(6):12-22,38,12.基金项目
农业农村部盐碱土改良与利用(干旱半干旱区盐碱地)重点实验室开放基金(YJDKFJJ202403) （干旱半干旱区盐碱地）
新疆生产建设兵团自然科学支持计划创新团队项目(2025DB012) （2025DB012）
新疆维吾尔自治区科学技术厅天山英才培养计划项目(2022TSYCLJ0039) （2022TSYCLJ0039）
国家自然科学基金项目(32301956) （32301956）
