干旱地区农业研究2025，Vol.43Issue(6)：57-64,76,9.DOI:10.7606/j.issn.1000-7601.2025.06.06
VrWRKY50基因生物信息学分析及调环酸钙缓解绿豆盐碱胁迫表达分析
Bioinformatics analysis of VrWRKY50 gene and expression analysis of prohexadione-calcium alleviating saline-alkali stress in mung bean
摘要
Abstract
To explore the role of VrWRKY50 gene in mung bean response to saline-alkali stress,analyze its protein structure and expression pattern under salt-alkali stress and prohexadione-calcium spray,in this study,17 WRKY50 homologous proteins were analyzed by bioinformatics.qRT-PCR was used to determine the expression pattern of the VrWRKY50 gene in mung bean under saline-alkali stress and prohexadione-calcium spray.The results showed that all 17 WRKY50 proteins were hydrophilic proteins,and Motif 1 was the main conserved motif of WRKY50.The WRKY50 gene contained 3～4 exons,and the promoter region contained 9 cis-acting elements related to growth and development,stress response,hormone response,etc.The protein homology of mung bean VrWRKY50 and adzuki bean VaWRKY50 was the highest.Salt and alkali stress significantly up-regulated Vr-WRKY50 gene expression in mung bean,increasing 13.91-fold(6th day)and 7.15-fold(8th day)in leaves and roots,respectively.The peak time of VrWRKY50 gene expression was advanced to 1 day by spraying prohexadione-calcium.The expression levels in leaves and roots were 47.70 times and 3.52 times those in the saline-alkali con-trol,respectively.关键词
绿豆/VrWRKY50基因/系统发育/盐碱胁迫/调环酸钙/基因表达Key words
mung bean/VrWRKY50 gene/phylogeny/saline-alkali stress/prohexadione-calcium/gene ex-pression分类
农业科技引用本文复制引用
PANG Yuanyuan,LI Chen,JIANG Haipeng,WANG Qingyan,LIANG Xilong,FANG Shumei..VrWRKY50基因生物信息学分析及调环酸钙缓解绿豆盐碱胁迫表达分析[J].干旱地区农业研究,2025,43(6):57-64,76,9.基金项目
国家自然科学基金项目(32272225,32472238) （32272225,32472238）
黑龙江省自然科学基金项目(LH2023C079) （LH2023C079）
