干旱地区农业研究2025，Vol.43Issue(6)：65-76,12.DOI:10.7606/j.issn.1000-7601.2025.06.07
酸枣MYB基因家族鉴定及其响应干旱胁迫表达分析
Identification of MYB gene family in Ziziphus jujuba and expression analysis in response to drought stress
摘要
Abstract
Ziziphus jujuba has excellent drought resistance,and MYB transcription factors play an important role in plant response to drought stress.In order to investigate the drought-resistant mechanism of MYB transcription factors in Z.jujuba var.spinosa,the MYB gene family of Z.jujuba var.spinose was identified and analyzed by bioin-formatics methods.Combined with the transcriptome data under drought stress,the key genes in response to drought stress were screened to provide a basis for the cultivation of drought-resistant and high-quality Z.jujube varieties.The results showed that 62 MYB gene family members were identified in the whole genome of Z.jujuba var.spinosa,distributed on 12 chromosomes,and all of them contained the conserved Motif2 structural domain,which was main-ly located in the cytoplasm,nucleus,and chloroplasts.The MYB members of Z.jujuba var.spinosa,Z.jujuba cv.'Dongzao',and Z.jujuba cv.'Junzao'were classified into seven subgroups.A total of 4 361 pairs of syntenic gene pairs were identified in the whole genome of Z.jujuba var.spinosa,among which 4 pairs of syntenic gene pairs were found in MYB family members.There were 6 and 10 pairs of collinearity genes in the MYB gene family of Z.jujuba var.spinosa,Z.jujuba cv.'Junzao',and Z.jujuba cv.'Dongzao',respectively.In the promoter region of the MYB gene family of Z.jujuba var.spinosa,41 types of cis-acting elements related to light response,environmental stress,hormone response,etc.were identified,including abscisic acid-responsive element ABRE in response to drought stress and MYB-binding site element MBS.In the expression pattern of drought stress,the MYB members ZjsMYB14,ZjsMYB21,ZjsMYB26,ZjsMYB43,ZjsMYB51,and ZjsMYB53 were significantly expressed in different developmental periods of jujube,which might be the key drought-resistant genes.关键词
酸枣/MYB基因家族/干旱胁迫/生物信息分析/表达分析Key words
Ziziphus jujuba/MYB gene family/drought stress/bioinformatic analysis/expression analysis分类
农业科技引用本文复制引用
DING Xubin,MA Yaping,LIU Jingyuan,SHAN Lei,LI Sulong,SONG Lihua..酸枣MYB基因家族鉴定及其响应干旱胁迫表达分析[J].干旱地区农业研究,2025,43(6):65-76,12.基金项目
国家自然科学基金项目(32160360) （32160360）
2024年宁夏大学研究生创新项目(GXXM2024-6) （GXXM2024-6）
宁夏自然科学基金项目(2021AAC03110) （2021AAC03110）
