干旱地区农业研究2025，Vol.43Issue(6)：77-87,11.DOI:10.7606/j.issn.1000-7601.2025.06.08
甜樱桃HD-Zip基因家族鉴定及胁迫条件下的表达分析
Identification of HD-Zip gene family in Prunusavium L.and expression analysis under stress conditions
摘要
Abstract
Homodomain-leucine zipper(HD-Zip)proteins are essential for regulating plant growth and devel-opment and responding to adversity stress.Bioinformatics methods were used to identify the sweet cherry(Prunusa-vium L.)HD-Zip gene family members and analyze their physicochemical properties,secondary structure,con-served motifs,phylogeny,promoter elements,and gene structure.Based on the transcriptome data,the HD-Zip family members were mined for tissue specificity and expression patterns under stress conditions;meanwhile,the expression of HD-Zip genes in different tissues of sweet cherry was detected by qRT-PCR.The results showed that there are 15 members of the HD-Zip family,coding for amino acids ranging from 127 aa to 837 aa,and most of the members are predicted to be localized in the nucleus.The HD-Zip family proteins were hydrophilic and unstable proteins,the structure of the HD-Zip gene was relatively complex,the number of exons ranged from 1 to 18,and different conserved motifs were distributed in different family members.The phylogenetic tree showed that the HD-Zip family members were divided into four subfamilies,and the members of the same subfamily had similar structur-al composition and conserved motifs.There were cis-acting elements in the promoter region of HD-Zip genes for phytohormones such as growth hormone,gibberellin,and jasmonic acid,as well as for a variety of adversity stres-ses.The transcriptome results showed that PavHB7A was specifically expressed in fruits and pedicels,and the ex-pression level of PavHB7B in flowers was significantly higher than that in other tissues,up to 200 times.The ex-pression level of PavHB15 in young shoots was 27 times higher than that in mature leaves.qRT-PCR results showed that HD-Zip genes were differentially expressed in buds,roots,stems,leaves,and flowers.After drought treat-ment,the expression of PavHB7A was up-regulated in the roots and leaves of'CDR-1',which increased by 665.6%and 365.9%,respectively,indicating that the PavHB7A gene was involved in the response process of drought stress.关键词
甜樱桃/HD-Zip转录因子/基因表达/非生物胁迫Key words
Prunusavium L./HD-Zip transcription factor/gene expression/abiotic stress分类
农业科技引用本文复制引用
BA Guang,GONG Yanjing,LIU Haiyan,DENG Fei,WANG Hongming..甜樱桃HD-Zip基因家族鉴定及胁迫条件下的表达分析[J].干旱地区农业研究,2025,43(6):77-87,11.基金项目
天水师范学院产业支撑引导项目(CYZ2022-02) （CYZ2022-02）
天水市秦州区科学技术局科学技术研究与开发项目(2023-NCKJG-1058) （2023-NCKJG-1058）
