赣南医科大学学报2025，Vol.45Issue(11)：1069-1071,1105,4.DOI:10.3969/j.issn.2097-7174.2025.11.007
诺如病毒快速免疫层析检测效能的评估及应用
Evaluation of the detection performance and application for rapid immunochromatographic test of norovirus
WU Yan1
作者信息
- 1. Center for Disease Control and Prevention of Xunyang District,Jiujiang,Jiangxi 332000
- 折叠
摘要
Abstract
Objective:To evaluate detection performance of a norovirus immunochromatographic(IC)test kit and provide evidence for its practical application.Methods:A total of 100 diarrheal samples were collected and tested in parallel using the IC test kit and the RT-qPCR methods recommended by quarantine industry standards.The detection efficacy of the IC test kit for norovirus and its genotyping was assessed through statistical analysis of results from both methods.Results:The sensitivity,specificity,and concordance rate of the IC detection kit reached 96%,100%,and 97%,respectively.Compared with the standard detection methods,the McNemar chi-square value was 1.333,and the P-value was 0.248,indicating no statistical difference.The results of further detection of different norovirus genotypes showed that the IC detection kits demonstrated a sensitivity of 100.0%for the norovirus GⅠ.5,GⅡ.3,and GⅡ.7 genotypes,while the sensitivities for the GⅡ.4 and GⅡ.17 genotypes were 94.4%and 87.5%,respectively.Conclusion:The IC detection kit has high sensitivity and specificity,the detection results show high consistency with RT-qPCR detection,making it suitable as a screening method for norovirus in primary healthcare institutions.关键词
诺如病毒/免疫层析/检测效能Key words
Norovirus/Immunochromatographic/Detection performance分类
医药卫生引用本文复制引用
WU Yan..诺如病毒快速免疫层析检测效能的评估及应用[J].赣南医科大学学报,2025,45(11):1069-1071,1105,4.
