临床与病理杂志2025，Vol.45Issue(9)：1122-1132,11.DOI:10.11817/j.issn.2095-6959.2025.250449
ADAMTSL3蛋白在3种不同类型的AKI模型小鼠中的表达
Expression of ADAMTSL3 protein in 3 different types of acute kidney injury mouse models
摘要
Abstract
Objective:Acute kidney injury(AKI)is a common and life-threatening clinical condition.At present,early,precise,and non-invasive diagnostic and therapeutic approaches remain insufficient.It is therefore essential to further investigate the underlying mechanisms of AKI onset and progression and to identify more specific and reliable targets.This study aims to the expression level and distribution of an adisintegrin-like and metalloprotease domain with thrombospondin type I motifs-like-3(ADAMTSL3)in 3 mouse models of AKI induced by ischemia/reperfusion(I/R),cisplatin(Cis),and folic acid(FA). Methods:Twenty-four male C57BL/6 mice were randomly divided into 4 groups(n=6 per group):Control(Sham),I/R,Cis,and FA groups.After model establishment,serum creatinine(SCr)and blood urea nitrogen(BUN)were measured.Kidney tissues were harvested for sectioning and subjected to hematoxylin and eosin(HE)staining,periodic acid Schiff(PAS)staining,Masson's trichrome staining,terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling(TUNEL)staining,immunofluorescence staining and Western blotting to assess renal injury and ADAMTSL3 protein expression. Results:Compared with the Sham group,SCr and BUN levels in all 3 AKI model groups(I/R,Cis,and FA)were significantly elevated(all P<0.05).Tubular injury was more severe,collagen fibrosis,glycogen deposition,and apoptosis were increased,and ADAMTSL3 expression was markedly upregulated in all AKI groups(all P<0.05),predominantly localized in renal tubules and interstitium.Moreover,ADAMTSL3 expression in the Cis and FA groups was both significantly higher than that in the I/R group(both P<0.05). Conclusion:All 3 AKI mouse models are successfully established.ADAMTSL3 protein expression is significantly increased in the kidneys of AKI mice,suggesting injury-associated upregulation and indicating its potential as a locational marker of renal parenchymal damage.ADAMTSL3 may play an important role in the mechanisms underlying AKI development,and targeting ADAMTSL3 can contribute to the development of early,non-invasive,and precise diagnostic biomarkers and therapeutic agents for AKI.关键词
整合素样金属蛋白酶结构域含血小板反应蛋白I型基序样3/急性肾损伤/急性肾损伤小鼠模型/缺血再灌注/顺铂/叶酸Key words
adisintegrin-like and metalloprotease domain with thrombospondin type I motifs-like-3/acute kidney injury/acute kidney injury mouse model/ischemia/reperfusion/cisplatin/folic acid引用本文复制引用
Wulaer·ADELI,LIN Lanyan,HUANG Xuan,CHU Xueqian,CHEN Sisi,LI Suhua..ADAMTSL3蛋白在3种不同类型的AKI模型小鼠中的表达[J].临床与病理杂志,2025,45(9):1122-1132,11.基金项目
国家自然科学基金(82360139) （82360139）
"天山英才"医药卫生高层次人才培养计划领军人才项目(TSYC202301A010) （TSYC202301A010）
新疆医科大学大学生创新创业训练计划项目(X202410760091).This work was supported by the National Natural Science Foundation(82360139),the"Tianshan Yingcai"Leading Talent Program of High-level Health Professionals Training Project in Medicine and Health(TSYC202301A010),and the Xinjiang Medical University College Student Innovation and Entrepreneurship Training Program(X202410760091),China. （X202410760091）
