贵州农业科学2025，Vol.53Issue(12)：72-81,10.DOI:10.3969/j.issn.1001-3601.2025.12.009
6类不同作用特点杀菌剂对三七黑斑病菌的毒力研究
Toxicity of 14 Fungicides with Six Different Functional Characteristics Against Black Spot Pathogen of Panax notoginseng
摘要
Abstract
[Objective]The antibacterial effect of 14 common fungicides against black spot pathogen of Panax notoginseng was investigated to provide the basis for controlling black spot of P.notoginseng and other Chinese medicinal materials.[Method]The pathogenicity of the pathogen causing black spot of P.notoginseng isolated from the diseased P.notoginseng leaves was detected,and the morphology and molecular biology of the isolated pathogen were identified.The antibacterial rate of 14 fungicides with six different functional characteristics(mitochondrial respiratory inhibitor,broad-spectrum fungicide,systemic fungicide,protective fungicide,sterol biosynthesis inhibitor and microbial antibiotic)against black spot pathogen of P.notoginseng was investigated by a antibacterial test.The regression equations for daily toxicity of 14 fungicides were calculated by using mycelium growth assay.The antibacterial effect of 14 fungicides was compared based on their EC50 dynamic change values.[Result]The pathogen causing black spot of P.notoginseng was identified as Alternaria alternata.The colony diameter treated with different fungicides increased with extension of antibacterial test days and the inhibition rate showed a declining trend generally.The correlation coefficient(r)of toxicity regression equations of different fungicides was more than 0.9,which indicated that the fungicide concentration was positively related to the inhibition rate very significantly.The EC50 value of 14 fungicides on 9 d was Fluazinam<Procymidone<Propiconazol<Tebuconazole<Difenoconazole<Dimethachlone<Boscalid<Hymexazol<Pyraclostrobin<Carbendazim<Mancozeb<Polyoxin<Fernasan<Ethylicin.The EC50 value of mitochondrial respiratory inhibitors was less than 10 mg/L and the inhibition rate of Boscalid,Pyraclostrobin and Fluazinam against A.alternata was 0.39％-54.95％,8.75％-45.14％and 57.27％-86.95％respectively.The EC50 value of broad-spectrum fungicides was 17.730 1-965.000 0 mg/L and the inhibition rate of Thiram,Mancozeb and Ethylicin against A.alternata was 6.21％-34.16％,5.64％-100.00％and 1.59％-7.83％separately.The EC50 value of internal fungicides was less than 20 mg/L and the inhibition rate of Dimethachlone,Hymexazol and Carbendazim against A.alternata was 21.17％-100.00％,0.56％-47.91％and 11.82％-35.38％respectively.The EC50 value of protective fungicides was less than 1 mg/L and the inhibition rate of saprophyl against A.alternata was 36.25％-82.50％.The EC50 value of sterol biosynthesis inhibitors against A.alternata was less than 1 mg/L and the inhibition rate of Difenoconazole,Tebuconazole and Propiconazol was 6.91％-88.10％,5.98％-61.08％and 13.38％-82.16％separately.The EC50 value of antibiotic fungicides derived from microbial source was less than 20 mg/L and the inhibition rate of Polyoxin against A.alternata was 0.38％-25.85％.[Conclusion]The antibacterial effect of sterol methylation inhibitors and protective fungicide with EC50<1 mg/L and mitochondrial respiratory inhibitors with EC50<10 mg/L against A.alternata causing black spot of P.notoginseng is better than other 3 types of fungicides.Fluazinam,Dimethachlone,Procymidone,Difenoconazole,Tebuconazole and Propiconazol with EC50<1 mg/L and Boscalid,Pyraclostrobin and Hymexazol with EC50 of 1-10 mg/L can be used for field control of black spot disease in P.notoginseng cultivation.关键词
三七/黑斑病/杀菌剂/菌丝生长速度/毒力测定/线粒体呼吸抑制剂/甾醇生物合成抑制剂Key words
Panax notoginseng/Alternaria alternata/fungicide/mycelial growth rate/toxicity determination/mitochondrial respiratory inhibitor/sterol biosynthesis inhibitor分类
农业科技引用本文复制引用
LI Xueping,TAO Yonghong,QU Yonghan,NONG Quandong,CHEN Xianmei,NONG Chuanjiang,RU Ruihong,LI Chunqing,YANG Yuling,YANG Yun..6类不同作用特点杀菌剂对三七黑斑病菌的毒力研究[J].贵州农业科学,2025,53(12):72-81,10.基金项目
中央农业产业发展资金项目"云南三七优势特色产业集群——三七仿野生栽培关键技术研究与应用示范"(文财农[2024]52号),"以三七为主的中医药产业链关键技术研究"(文财农[2023]10号),"绿色高效三七种植技术提升与应用示范"(文财农[2024]3号) （文财农[2024]52号）
云南省乡村振兴科技专项"服务型科技特派员"(云财教[2023]31号) （云财教[2023]31号）
