广州医药2025，Vol.56Issue(11)：1482-1490,1500,10.DOI:10.20223/j.cnki.1000-8535.2025.11.003
GRB14基因在肺腺癌中的表达及对预后的影响
Expression and clinical significance of GRB14 in lung adenocarcinoma
摘要
Abstract
Objective To assess the specific mechanism of growth factor receptor-bound protein 14(GRB14)in the prognosis of lung adenocarcinoma(LUAD)patients.Methods The expression of GRB14 mRNA in LUAD and normal lung tissue was explored using TIMER database,UALCAN database,and GEPIA database.The expression of GRB14 protein was examined by immunohistochemistry using a tissue microarray.Then,the associations of GRB14 expression with clinicopathological features and clinical outcomes of LUAD were validated by analyzing TCGA database at the mRNA level and statistically evaluating the results.TIMER database was used to analyze immune infiltration of GRB14 in LUAD.Protein-protein interaction of GRB14 were analyzed using the String database.Results Using the TIMER database,we found that GRB14 mRNA was highly expressed in various solid tumors and LUAD tissues compared to normal tissues(P<0.05).Comparing with the normal group,the expression of GRB14 was increased in LUAD(P<0.01)via using UALCAN database and GEPIA database.The expression level of GRB14 protein in the LUAD tissues was significantly higher than that in the noncancerous LUAD tissues(LUAD[6.07±1.01]vs benign,[4.80±1.22];P<0.01)in tissue microarray.Median overall survival in the high and low GRB14 expression groups in LUAD was(41.59±5.2)and(88.67±16.69)months respectively.We plotted the ROC curves of 3-year survival rate and 5-year survival rate which again suggested that the model had good predictive performance.Univariate analysis revealed that individual cancer stages(Ⅲ-Ⅳ)(P<0.01),tumor(T3-4)(P<0.01),lymph node metastasis(N1-3)(P<0.05)and GRB14 expression(P<0.01)were risk factors affecting the median overall survival time of LUAD.According to Cox multiple regression analysis,we found that lymph node metastasis(N1-3)(P<0.05)and GRB14 expression(P<0.01)were risk factors affecting the median overall survival time of LUAD.Using TIMER database analysis,the mRNA level of GRB14 was significantly correlated with macrophages(r=-0.164,P<0.01),neutrophils(r=-0.175,P<0.01)and dendritic cells(r=-0.148,P<0.01).Through analysis of the String database,it was found that proteins that interacted with GRB14 including EGFR,HRAS,FGFR1,INSR,CNGA1,COBLL1,LYPLAL1,TNKS2,TNKS,PRKCZ.Conclusions The results of the present study suggest that GRB14 may efficiently predict poor survival in LUAD patients.关键词
生长因子受体结合蛋白14/肺腺癌Key words
growth factor receptor-bound protein 14/lung adenocarcinoma引用本文复制引用
CHEN Qixian,HE Weiguo,HE Limei,LIANG Zhike,XU Haoming,GUO Mingyi,LIU Zhaohui..GRB14基因在肺腺癌中的表达及对预后的影响[J].广州医药,2025,56(11):1482-1490,1500,10.基金项目
国家自然科学基金项目(82370552) （82370552）
