广州医药2025，Vol.56Issue(11)：1491-1500,10.DOI:10.20223/j.cnki.1000-8535.2025.11.004
基于孟德尔随机化以及Meta分析方法评估CX3CL1表达水平与系统性红斑狼疮的因果关系
Causal relationship between CX3CL1 expression levels and systemic lupus erythematosus based on Mendelian randomization and Meta-analysis
摘要
Abstract
Objective To investigate the causal relationship between CX3CL1 levels and the risk of systemic lupus erythematosus(SLE)using two-sample Mendelian randomization and Meta-analysis methods.Methods Genome-Wide Association Study(GWAS)data for CX3CL1 levels and SLE were obtained.Single nucleotide polymorphisms(SNPs)were used as instrumental variables,and sensitive SNPs were selected for analysis.Two-sample Mendelian randomization was performed using the inverse variance weighted(IVW)method,weighted median(WM)method,and MR-Egger regression to evaluate the causal relationship between CX3CL1 levels and SLE,with OR values assessing this relationship.Heterogeneity and pleiotropy tests were conducted on the results.Meta-analysis was performed using the Meta package in R software,and colocalization analysis was conducted using the coloc package.Results Nine SLE outcomes were included as outcome variables,with four variables(ebi-a-GCST90018917[OR=2.14,95%CI:1.50-3.06],ebi-a-GCST003156[OR=2.25,95%CI:1.00-5.06],ebi-a-GCST90014238[OR=3.02,95%CI:1.54-5.94],finn-b-SLE_NOS[OR=1.81,95%CI:1.01-3.22])indicating a causal relationship between CX3CL1 expression levels and SLE.The forest plot for OR 95%CI showed that CX3CL1 expression levels in SLE patients were significantly higher than in healthy individuals(OR=1.87[95%CI:1.53-2.29],P＜0.001).Colocalization analysis suggested that there was shared genetic variation sites(rs170364)between CX3CL1 expression levels and SLE phenotype.Conclusions There is a positive causal relationship between CX3CL1 expression levels and SLE,with increased CX3CL1 levels elevating the risk of developing SLE.关键词
CX3CL1表达水平/系统性红斑狼疮/孟德尔/Meta分析Key words
CX3CL1 expression levels/systemic lupus erythematosus/Mendelian randomization/Meta-analysis引用本文复制引用
DONG Cuicui,ZHAO Zhihu..基于孟德尔随机化以及Meta分析方法评估CX3CL1表达水平与系统性红斑狼疮的因果关系[J].广州医药,2025,56(11):1491-1500,10.基金项目
国家自然科学基金青年基金(82302701) （82302701）
天津市卫生健康委科技项目面上基金项目(TJWJ2022MS025)及青年基金项目(TJWJ2022QN051) （TJWJ2022MS025）
天津市科技局项目(22JCQNJC01060,22ZYJDSY00110) （22JCQNJC01060,22ZYJDSY00110）
