海南医科大学学报2025，Vol.31Issue(23)：1761-1769,9.DOI:10.13210/j.cnki.jhmu.20250612.001
滨海村庄多环境介质与居民肠道菌群的耐药、毒力基因及KEGG通路特征分析
Analysis of antibiotic resistance,virulence genes and KEGG pathway characteristics of multi-environmental media and intestinal flora in coastal villages
摘要
Abstract
Objective:To observe the distribution and correlation characteristics of antibiotic resistance genes,virulence genes and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway in human and multi-environmental media bacteria in coastal communities,and to provide evidence for further exploration of the interaction,mechanism and risk prevention between human and environmental microorganisms,based on the"One Health"strategy.Methods:Human feces,beach and pig farm soil samples were collected aseptically.The second generation sequencing was performed in Majorbio Bio-Pharm Technology Co.,Ltd.by shotgun metagenic method,annotation was performed by Comprehensive Antibiotic Resistance Database,Virulence Factors Data-base and KEGG database,and differential pathway analysis was performed by Linear Discriminant Analysis Effect Size and KEGG pathway enrichment method.The Spearman test was used for non-parametric correlation analysis.Results:Multiple antibi-otic resistances,tetracycline,macrolides,glycopeptides,and quinolones were mostly correlated with the human body and beach,soil(r=0.573-0.783,P<0.05),especially tetracycline and glycopeptides,but there was no correlation with the abundance of ami-noglycosides and polymyxin resistance genes on the beach(P>0.05).Linear Discriminant Analysis(LDA)indicated that the most common drug resistance mechanisms were antibiotic target change,target protection and decreased permeability.Beach A was the mechanism of antibiotic efflux pump,and the soil of pig farm was mainly replaced by antibiotic target(LDA>4).The de-fensive virulence factor was more significant in human intestinal microbe.Attack virulence factors and non-specific virulence factors were more significant in Beach B(LDA>4).Two types of virulence genes strongly associated with beaches in human gut bacteria were defensive and regulatory virulence factors(r=0.727‒0.840,P<0.05).KEGG enrichment analysis showed that the three KEGG Orthology pathways of human flora were the phosphotransferase system(PTS),galactose,and ribosome,and only one PTS was more abundant in the human compared to the pig farm soil.The higher microbial pathways were carbon metabolism,methane metabolism,amino acid metabolism,and fatty acid metabolism,respectively.Escherichia coli biofilm had more up-regulated pathways in humans,while Pseudomonas aeruginosa and Vibrio cholerae biofilm had more up-regulated pathways in environment.Conclusion:The strong correlation of tetracycline and glycopeptide resistance between human and environment sug-gesting that it may be common or unreasonable usage of the antibiotics in human and animal.The strong correlation between two types of virulence factors in human and beach environmental flora.Differences in KEGG pathways were more pronounced between beach and human microbiomes than between soil and human microbiomes.It is necessary to further explore the interaction between the coastal environment and humans and animals from the perspective of One Health.关键词
滨海村庄/耐药基因/毒力基因/KEGG通路/One HealthKey words
Coastal village/Drug resistance gene/Virulence gene/KEGG channel/One Health分类
医药卫生引用本文复制引用
ZHANG Yu,GAO Yi,PENG Xiaojie,YUAN Xinyu,CHEN Yaye,LONG Wenfang..滨海村庄多环境介质与居民肠道菌群的耐药、毒力基因及KEGG通路特征分析[J].海南医科大学学报,2025,31(23):1761-1769,9.基金项目
This study was supported by the Key Research and Development Project of Hainan Province(ZDYF2020181) 海南省重点研发项目(ZDYF2020181) （ZDYF2020181）
