海南医科大学学报2025，Vol.31Issue(23)：1770-1777,8.DOI:10.13210/j.cnki.jhmu.20250331.003
基于HIF-1α/TGF-β1/Smad3通路探讨顾步汤治疗糖尿病足溃疡的作用机制
Exploring the mechanism of Gubu Decoction in treating diabetes foot ulcer based on HIF-1α/TGF-β1/Smad3 pathway
摘要
Abstract
Objective:To explore the mechanism of Gubu Decoction(GBD)in improving the healing of diabetes foot ulcer(DFU)by regulating HIF-1α/TGF-β1/Smad3 pathway.Methods:The C57BL/6J mouse DFU model was constructed and ran-domly divided into a model group and GBD low,medium,and high dose groups,with 10 mice in each group.Another 10 mice were selected as the blank group,and all mice were treated continuously for 14 days.After administration,H&E staining and Mas-son staining were used to observe the pathological morphology and collagen deposition of ulcer tissue.EdU labeled immunofluores-cence was used to detect fibroblast proliferation in ulcer tissue,TUNEL was used to detect fibroblast apoptosis in ulcer tissue,ELISA was used to detect the levels of inflammatory factors(TNF-α,IL-6,IL-10)and oxidative stress(MDA,SOD,GSH-PX)in ulcer tissue,and Western blot was used to detect the expression of HIF-1α/TGF-β1/Smad3 pathway related pro-teins.Results:Compared to the model group,GBD significantly reduced inflammatory cell infiltration in ulcer tissue,increased the number of collagen fibers,granulation tissue,and newly formed capillaries in ulcer tissue,reduced fibroblast proliferation rate,decreased ROS positive cell rate,fibroblast apoptosis,IL-6,TNF-α levels,MDA content,HIF-1α,TGF-β1,TβR1,and p-Smad3 protein expression,increased IL-10 levels,SOD,and GSH-PX content(P<0.01).Conclusion:GBD can reduce DFU inflammation and oxidative stress levels,activate the HIF-1α/TGF-β1/Smad3 pathway,promote fibroblast generation,and im-prove ulcer tissue healing.关键词
顾步汤/糖尿病足溃疡/HIF-1α/TGF-β1/Smad3信号通路/创面愈合Key words
Gubu Decoction/Diabetic foot ulcers/HIF-1α/TGF-β1/Smad3 signaling pathway/Wound healing分类
医药卫生引用本文复制引用
WANG Jixue,YANG Xirui,ZHOU Tao,YAN Shuxun,WANG Siqi,XIA Lianheng,YUAN Xingxing..基于HIF-1α/TGF-β1/Smad3通路探讨顾步汤治疗糖尿病足溃疡的作用机制[J].海南医科大学学报,2025,31(23):1770-1777,8.基金项目
This study was supported by the National Natural Science Foundation of China(82205195,82205117) （82205195,82205117）
Henan Province Postdoctoral Research Project(HN2024080,HN2024083) （HN2024080,HN2024083）
Henan Province Medical Science and Technology Key Project(LHGJ2024067,LHGJ20230686) （LHGJ2024067,LHGJ20230686）
Research Special Project of Henan Province Clinical Research Base of Traditional Chinese Medicine(2022JDZX127,2022JDZX135) 国家自然科学基金(82205195,82205117) （2022JDZX127,2022JDZX135）
河南省博士后科研项目(HN2024080,HN2024083) （HN2024080,HN2024083）
河南省医学科技攻关计划项目(LHGJ2024067,LHGJ20230686) （LHGJ2024067,LHGJ20230686）
河南省中医临床研究基地科研专项(2022JDZX127,2022JDZX135) （2022JDZX127,2022JDZX135）
