海南医科大学学报2025，Vol.31Issue(23)：1778-1785,8.DOI:10.13210/j.cnki.jhmu.20240728.001
基于16S rDNA高通量测序研究电针对控制性超排卵小鼠肠道菌群的调节作用
Modulation of intestinal microbiota in mice with controlled ovarian hyperstimulation by electroacupuncture:A study based on 16S rDNA high-throughput sequencing
摘要
Abstract
Objective:To analyze the effects of electroacupuncture on the relative abundance and diversity of intestinal microbi-ota species in controlled ovarian hyperstimulation(COH)mice through 16S rDNA high-throughput sequencing,as well as to in-vestigate its potential mechanism in enhancing the clinical pregnancy rate of in vitro fertilization-embryo transplantation.Methods:A total of 27 cyclic regular female mice were randomly divided into the natural cycle group,the COH group,and the electroacu-puncture group,with 9 mice in each group.The animal model was prepared using a controlled ovarian hyperstimulation protocol.In the electroacupuncture group,electroacupuncture treatment was performed on the day of human chorionic gonadotropin injec-tion,targeting the Guanyuan,Zhongji,and Sanyinjiao acupoints with continuous waves at a frequency of 2 Hz,once a day for 15 min each time.The natural cycle group and COH group received no intervention.On the 4th day of pregnancy(the implantation window),fecal samples from each group of mice were collected for 16S rDNA high-throughput sequencing,followed by analysis of species relative abundance,alpha diversity,beta diversity,and predicted functional metabolism.Results:The numbers of oper-ational taxonomic units in the natural cycle group,COH group,and electroacupuncture group were 1 083,1 135,and 1 125,re-spectively.Compared to the natural cycle group,the relative abundance of Bacteroidota,Verrucomicrobiota,Parabacteroides,Bacteroides,and Alloprevotellain the COH group were increased,and the relative abundance of Firmicutes,Lactobacillus,and Alistipes were decreased.After the treatment of electroacupuncture,the above-mentioned microorganisms showed different de-grees of improvement in relative abundance.Compared to the natural cycle group,the Shannon,Simpson,and Chao1 indices of the COH group increased;compared to the COH group,the Shannon and Chao1 indices of the electroacupuncture group de-creased,the difference were not statistically significant(P>0.05).The functional prediction analysis found that electroacupunc-ture could regulate the functional genes involved in carbohydrate metabolism and amino acid metabolism.Conclusion:The admin-istration of exogenous hormones alters the structural equilibrium of intestinal microbiota in mice,while electroacupuncture demon-strates the capability to regulate both the relative abundance of intestinal microbiota species and the overall diversity of microbiota in mice undergoing controlled ovarian hyperstimulation.关键词
控制性超排卵/电针/肠道菌群/高通量测序/功能预测Key words
Controlled ovarian hyperstimulation/Electroacupuncture/Intestinal microbiota/High-throughput sequencing/Functional prediction分类
医药卫生引用本文复制引用
ZHANG Lei,LIU Rui,LIU Xiaoya,MING Hao,WANG Dong,ZHU Shuxiu..基于16S rDNA高通量测序研究电针对控制性超排卵小鼠肠道菌群的调节作用[J].海南医科大学学报,2025,31(23):1778-1785,8.基金项目
This study was supported by the National Natural Science Foundation of China(81674060) （81674060）
Research Fund of Jianghan University(2021yb142) 国家自然科学基金(81674060) （2021yb142）
江汉大学校级科研项目资助计划(2021yb142) （2021yb142）
