海南医科大学学报2025，Vol.31Issue(23)：1786-1792,7.DOI:10.13210/j.cnki.jhmu.20241220.002
负载奥沙利铂的PLGA微粒大鼠体内外安全性评估
In vitro and in vivo safety assessment of oxaliplatin-loaded PLGA microparticles in rats
摘要
Abstract
Objective:To investigate the biosafety of poly lactic-co-glycolic acid(PLGA)microparticles loaded with oxaliplat-in in rats in vitro and in vivo,and to verify the feasibility of injection of PLGA microparticles loaded with oxaliplatin.Methods:The effect of microparticles on the proliferation rate of mouse epithelioid fibroblast cells L929 was detected by CCK-8 to evaluate its in vitro safety.The microspheres were injected into the right posterior calf muscle of 200‒250 g SD female rats.Routine blood tests(WBC,HGB,PLT,and PCT)were performed,and blood biochemical indicators(ALT,AST,TP,ALB,ALP,BUN,Cre,CHO,and TBIL)were measured.Major organs'(liver,kidneys,bone marrow,and muscle)pathological sections were stained using H&E staining.The biosafety of the nanoparticles was verified in vivo.Results:In vitro cell proliferation experi-ments showed that PLGA drugs loaded with oxaliplatin may exhibit certain cytotoxicity due to the function of sustained release of oxaliplatin.In vivo experiments showed no obvious chronic toxicity in the rats.Pathological tissue section of H&E staining did not prompt important chronic tissue damage.Conclusion:PLGA particle loaded with oxaliplatin meets the requirements for injection,does not cause damage to the injection site,and does not cause obvious acute or chronic side effects after injection,which has a high safety,and is expected to be applied in clinical practice to provide a new method for the treatment of gastric cancer.关键词
PLGA/奥沙利铂/体内外实验/安全性Key words
PLGA/Oxaliplatin/Experiments in vivo and in vitro/Safety分类
医药卫生引用本文复制引用
JIAO Binqi,CAO Kun,ZHANG Junwei,CHEN Mingzhe,ZHANG Yanzhi,WANG Yi,LI Fukang,ZHANG Hao..负载奥沙利铂的PLGA微粒大鼠体内外安全性评估[J].海南医科大学学报,2025,31(23):1786-1792,7.基金项目
This study was supported by the Hainan Health Science and Technology Innovation Joint Project(WSJK2024MS222) 海南省卫生健康科技创新联合项目(WSJK2024MS222) （WSJK2024MS222）
