海南医科大学学报2025，Vol.31Issue(23)：1793-1801,9.DOI:10.13210/j.cnki.jhmu.20250102.003
益肺通络方对肺纤维化模型大鼠IL-17A影响的研究
Effect of Yifei Tongluo Formula regulating IL-17A on pulmonary fibrosis model rats
摘要
Abstract
Objective:To explore the effect of Yifei Tongluo Formula on regulating IL-17A in the model rats,constructing an animal model of pulmonary fibrosis using bleomycin tracheal instillation method.Methods:SD rat pulmonary fibrosis model was constructed using bleomycin tracheal instillation method,and randomly divided into the control group,the model group,the pred-nisone group,the low-dose Yifei Tongluo Formula group,and the high-dose Yifei Tongluo Formula group.Corresponding inter-ventions were given for 14 days,and rat serum and lung tissue were taken for testing.The degree of pulmonary fibrosis in rats was evaluated by morphological observation of lung tissue,MicroCT imaging observation,H&E staining,and Masson staining.Im-munohistochemistry was used to analyze the expression of pulmonary fibrosis related proteins such as α-smooth muscle actin(α-SMA)and vimentin protein(Vimentin).Immunofluorescence was used to detected IL-17A and other signaling molecule ex-pressions.ELISA was used to detect the secretion level of hydroxyproline(HYP)in rat serum and the content of cytokines such as IL-17A.PCR was used to detect the relative expression of IL-17A mRNA.Western Blotting was used to detect the expression of IL-17A protein.Results:Observation of the morphology of rat lung tissue shows that Yifei Tongluo Formula has a significant im-provement effect on the appearance,color,lung lobe contour,and volume elasticity of both lungs in rats.Observation of chest Mi-croCT imaging showed that Yifei Tongluo Formula can reduce the degree of lung inflammation and improve fibrosis symptoms in rats.H&E staining of rat lung tissue showed that Yifei Tongluo Formula can alleviate pathological features such as inflammatory cell infiltration in lung tissue.Masson staining of rat lung tissue showed that Yifei Tongluo Formula can improve fibrosis and tissue proliferation in lung tissue.The immunohistochemical results showed that compared to the control group,the average positive area of α-SMA and Vimentin in the lung tissue of the model group rats was significantly increased(P<0.05).Compared to the model group,the average positive area of α-SMA in the lung tissue of rats in the Yifei Tongluo Formula group was significantly reduced(P<0.01),especially after high-dose Yifei Tongluo Formula intervention,the average positive area of Vimentin in the lung tissue of rats was significantly reduced(P<0.05).Immunofluorescence images showed that compared to the control group,the IL-17A fluorescence intensity in the lung tissue of the model group rats was higher,and the fluorescence intensity was significantly reduced after intervention Yifei Tongluo Formula.The ELISA results showed that compared to the control group,the HYP and IL-17A levels in the model group rats were significantly increased(P<0.05).Compared to the model group,the levels of the above-mentioned cytokines in the Yifei Tongluo Formula group of rats were significantly reduced(P<0.05).The PCR results showed that compared to the control group,the model group rats significantly upregulated the relative expression of IL-17A mRNA(P<0.05).Compared to the model group,the relative expression of IL-17A mRNA in the Yifei Tongluo Formula group rats was significantly reduced(P<0.05).The Western blot test results showed that compared to the control group,the expression of IL-17A protein in the lung tissue of the model group rats was significantly increased(P<0.05).Compared to the model group,Yifei Tongluo Formula could significantly inhibit the expression of IL-17A protein(P<0.05).Conclusion:Yifei Tongluo Formu-la has a protective effect on bleomycin induced pulmonary fibrosis in rats.Yifei Tongluo Formula may improve rat pulmonary fibro-sis by downregulating IL-17A.关键词
肺纤维化/益肺通络方/IL-17A/实验研究Key words
Pulmonary fibrosis/Yifei Tongluo Decoction/IL-17A/Experimental study分类
医药卫生引用本文复制引用
MA Xian,FENG Jiateng,LI Zefeng,ZHOU Bowen,LIU Huaman,XU Fei,XIN Lingjie,JIA Xinhua..益肺通络方对肺纤维化模型大鼠IL-17A影响的研究[J].海南医科大学学报,2025,31(23):1793-1801,9.基金项目
This study was supported by the 2022 Young Qihuang Scholar Training Program[Guo Zhong Yao Ren Jiao Han (2022)No.256] （2022）
Shandong Province High-level Talent Cultivation Project for Traditional Chinese Medicine[Lu Wei Han(2023)No.143] 2022年青年岐黄学者培养项目[国中医药人教函(2022)256号] （2023）
山东省中医药高层次人才培育项目[鲁卫函(2023)143号] （2023）
