经济动物学报2025，Vol.29Issue(6)：481-484,4.DOI:10.13326/j.jea.2025.1842
磁珠法和柱提法从天然麝香仁中提取基因组DNA的比较
Comparison of Magnetic Bead Method and Spin Column Method for Extracting Genomic DNA from Natural Musk
摘要
Abstract
To compare the efficacy of magnetic bead-based and column-based method for extracting genomic DNA from Moschus grains,DNA was extracted from natural musk grains of varying masses using respective commercial kits.The quality concentration,purity,and integrity of the DNA obtained by both methods were compared using agarose gel electrophoresis and an ultra-micro nucleic acid analyzer.The utility of the extracted DNA was further assessed via conventional PCR and real-time fluorescent PCR.The results demonstrated that both methods successfully extracted genomic DNA even from the smallest sample mass(30 mg),and the DNA was suitable for PCR amplification of small target gene fragments.When the sample mass was≥50 mg,the DNA quality obtained by the magnetic bead-based method was slightly superior to that obtained by the column-based method.This study indicates that both extraction methods are capable of isolating DNA from natural musk grains,and the extracted DNA is suitable for detecting forest musk deer(Moschus berezovskii)-derived components using real-time fluorescent PCR.The selection of method can be made based on practical considerations.关键词
麝香/DNA提取/磁珠法/柱提法/实时荧光定量PCRKey words
musk/DNA extraction/magnetic bead method/spin column method/real-time PCR分类
农业科技引用本文复制引用
ZHOU Lei,DU Rongsheng,ZHANG Haimei,CHEN Feng,XIAN Siwei,WU Jie..磁珠法和柱提法从天然麝香仁中提取基因组DNA的比较[J].经济动物学报,2025,29(6):481-484,4.基金项目
四川省药品监督管理局科技计划项目(202104) （202104）
