菌物学报2025，Vol.44Issue(12)：21-29,9.DOI:10.13346/j.mycosystema.250289
新型分子分型技术推动隐球菌病的临床诊治水平
Novel molecular typing technology promotes advance in diagnosis and treatment level of cryptococcosis
摘要
Abstract
Cryptococcal infection often leads to fatal cryptococcal meningitis,with the primary pathogens being the Cryptococcus neoformans species complex(CNSC)and the Cryptococcus gattii species complex(CGSC).To date,at least eight cryptococcal lineages have been identified,which exhibit significant differences in geographic distribution and virulence characteristics.This review provides a detailed analysis of various molecular typing methods used in cryptococcal research,including serotyping,multi-locus enzyme electrophoresis(MLEE),pulsed-field gel electrophoresis(PFGE),random amplified polymorphic DNA(RAPD),restriction fragment length polymorphism(RFLP),PCR fingerprinting,amplified fragment length polymorphism(AFLP),multilocus microsatellite typing(MLMT),multilocus sequence typing(MLST),mass spectrometry identification(MALDI-TOF MS),and whole genome sequencing(WGS)techniques.In the future,combined application of MALDI-TOF MS,WGS and AI machine learning would improve our understandings of the epidemiological characteristics and evolutionary history of Cryptococcus,and reveal the phylogenetic patterns,origin location and transmission patterns of cryptococcal pathogens,as well as the micro-evolutionary mutations occurring within host niches,which are of great significance to clinical decisions on diagnosis and treatment of cryptococcosis.关键词
隐球菌病/新生/格特隐球菌菌种复合体/分子分型/流行病学Key words
cryptococcosis/Cryptococcus neoformans/Cryptococcus gattii species complex/molecular typing/epidemiology引用本文复制引用
HUANG Meilin,QI Boling,HONG Nan,CHEN Min..新型分子分型技术推动隐球菌病的临床诊治水平[J].菌物学报,2025,44(12):21-29,9.基金项目
国家自然科学基金(82172291,82102419) （82172291,82102419）
上海市浦东新区卫生健康委员会重点学科(PWZxk2025-01)This work was supported by the National Natural Science Foundation of China(82172291,82102419)and the Medical Discipline Construction Program of Shanghai Pudong New Area Health Commission(the Key Disciplines Program PWZxk2025-01). （PWZxk2025-01）
