菌物学报2025，Vol.44Issue(12)：60-69,10.DOI:10.13346/j.mycosystema.250136
须癣毛癣菌临床分离株的同宗配合现象
Homothallism in a clinical isolate of Trichophyton mentagrophytes
摘要
Abstract
This study aims at investigating whether Trichophyton mentagrophytes enhances its host adaptability through homothallic sexual reproduction.The clinical isolate JYP23487,obtained from the hair of a 3-year-old boy suffered from kerion lesion,was identified as T.mentagrophytes via ITS sequencing following purification.Morphological analysis revealed two distinct phenotypes,velvety type and granular type,with evidence of self-fertilization.After isolating these phenotypes individually,the MAT1-1-1 and MAT1-2-1 gene fragments were amplified for further analysis.Sequence chromatograms were compared,and a haplotype network diagram was constructed by referencing available data on T.mentagrophytes and related species harboring the MAT1-1-1 gene locus.Sequence comparison revealed that the granular type strain exhibited base doublets at common single nucleotide polymorphism(SNP)sites within the T.mentagrophytes complex population,indicating that this strain was heterozygous.Haplotype analysis of the MAT1-1-1 gene demonstrated that 30 strains of T.mentagrophytes and related species could be classified into four haplotypes.The heterozygous strain JYP23487k corresponded to the hypothetical intersection point between Hap 1 and Hap 2 in the haplotype networks.This finding fills a critical gap in the evolutionary continuum of the population and supports the genetic continuity inferred from haplotype analysis.The identification of this heterozygous strain provides evidence that besides colonizing and infecting humans through asexual reproduction,T.mentagrophytes also employs homothallic sexual reproduction as an adaptive strategy to explore potential host transfer and survive in diverse ecological niches.关键词
须癣毛癣菌复合体/交配型基因/单倍型分析/适应性/杂合Key words
Trichophyton mentagrophytes complex/mating-type gene/haplotype analysis/adaptability/heterozygosity引用本文复制引用
HU Wei,XU Kexin,CHE Di,JIANG Yanping..须癣毛癣菌临床分离株的同宗配合现象[J].菌物学报,2025,44(12):60-69,10.基金项目
贵州医科大学高层次人才启动基金(贵医大[2022]013 号)This work was supported by the Startup Fund for High-level Talents at Guizhou Medical University(Document No.[2022]013). （贵医大[2022]013 号）
