菌物学报2025，Vol.44Issue(12)：70-91,22.DOI:10.13346/j.mycosystema.250114
DNA条形码鉴定人类病原青霉
DNA barcoding for identification of human pathogenic Penicillium species
摘要
Abstract
Fungal species identification constitutes the cornerstone of medical mycology research,particularly in the diagnosis and treatment of pathogenic fungi.With the increase of diversity and quantity of clinical fungal pathogens,morphological identification has proven insufficient to meet the requirements of precise species determination.DNA barcoding,as a rapid species identification technology based on specific short genetic markers,enables accurate identification of clinical pathogens,particularly newly isolated fungal strains.This study integrated morphological characterization with DNA barcoding techniques to achieve species-level identification of 35 Penicillium strains isolated from patients with skin and skin structure fungal infections through combined phenotypic analysis and multi-locus phylogenetic assessment.As a result,these clinical isolates were classified into nine species:Penicillium allii-sativi,P.aurantiogriseum,P.brevicompactum,P.chrysogenum,P.crustosum,P.expansum,P.mexicanum,P.polonicum,and P.rubens.Of which P.allii-sativi and P.mexicanum were newly recorded in China and first globally isolated from clinical specimens.Our findings suggest that the integration of morphological and DNA barcoding approaches provides a feasible methodology for precise species-level identification of Penicillium isolates,potentially serving as a diagnostic technique for clinical identification of pathogenic Penicillium species.关键词
病原真菌/青霉/DNA条形码/菌种鉴定/真菌分类学/分子系统学Key words
pathogenic fungi/Penicillium/DNA barcoding/fungal identification/fungal taxonomy/molecular phylogenetics引用本文复制引用
XIAO Han,ZHANG Gongjie,JOS Houbraken,SUN Tingting,CHEN Ze,LI Dongming..DNA条形码鉴定人类病原青霉[J].菌物学报,2025,44(12):70-91,22.基金项目
国家重点研发计划(2022YFC2504800 to DML) （2022YFC2504800 to DML）
国家自然科学基金(32070019,31770013 to DML)This work was supported by the National Key Research and Development Program of China(2022YFC2504800 to DML)and the National Natural Science Foundation of China(32070019,31770013 to DML). （32070019,31770013 to DML）
