菌物学报2025，Vol.44Issue(12)：106-119,14.DOI:10.13346/j.mycosystema.250029
一种皮炎外瓶霉外排泵编码基因及蛋白的功能
Function of a novel Exophiala dermatitidis efflux pump encoding gene and protein
摘要
Abstract
This study explored the biological function of the ABCB1 gene(ATP-binding cassette,subfamily B,member 1,GenBank ID:20312659)in Exophiala dermatitidis,focusing on its role in fungal growth and drug sensitivity.An ABCB1 knockout strain was constructed,and its phenotypic characteristics were systematically compared with those of the wild-type strain,including growth rate,drug sensitivity,drug efflux capacity,and intracellular ATP levels.The results demonstrated that the ABCB1 gene likely belongs to the MDR/TAP subfamily of ABC transporters and is involved in nutrient uptake and drug efflux processes.The knockout strain exhibited significantly reduction in growth rate,diminution of hyphal and conidial production,enhancement of susceptibility to azole antifungals,impairment of drug efflux capability,and decrease of intracellular ATP content.These findings indicate that the ABCB1 gene plays a crucial role in the growth and drug resistance of E.dermatitidis,suggesting its potential as a therapeutic target for novel antifungal drug development.关键词
ABC转运蛋白/ABCB1基因/药物敏感性/药物外排Key words
ATP-binding cassette transporter/ABCB1 gene/drug sensitivity/drug efflux引用本文复制引用
YUAN Mingzhu,ZHU Yutang,ZHANG Jie,LI Xiaotong,SUN Yi,GAO Lujuan..一种皮炎外瓶霉外排泵编码基因及蛋白的功能[J].菌物学报,2025,44(12):106-119,14.基金项目
福建省卫健委科技计划(2023GGB10) （2023GGB10）
湖北省重点研发计划(2024BCB043)This work was supported by the Fujian Provincial Health Commission Science and Technology Plan Project(2023GGB10)and the Key Research and Development Project of Hubei Province(2024BCB043). （2024BCB043）
