江西农业学报2025，Vol.37Issue(12)：69-78,10.DOI:10.19386/j.cnki.jxnyxb.2025.12.010
江西铅山红芽芋脱毒苗遗传稳定性的SRAP检测
SRAP Detection of Genetic Stability of Virus-free Seedlings of Jiangxi Yanshan Red Sprout Taro
摘要
Abstract
The genetic stability of shoot-tip conventional culture virus-free plantlets,shoot-tip virus-free plantlets by vitrification cryotherapy,shoot-tip virus-free plantlets by encapsulation vitrification cryotherapy,shoot-tip virus-free plantlets by encapsulation dehydration cryotherapy,and shoot-tip virus-free plantlets by droplet vitrification cryotherapy was tested using SRAP molecular marker technology,with field planted seedlings,callus regenerated plantlets,3-year plantlets subcultured at room temperature,and 5-year plantlets subcultured at room temperature of Jiangxi Yanshan red sprout taro as controls.The results showed that the optimal 12 pairs of primers for SRAP in Jiangxi Yanshan red sprout taro were P13,P19,P22,P24,P26,P34,P35,P36,P41,P44,P50 and P52.The 12 pairs of primers were used for PCR amplification of 9 samples of Jiangxi Yanshan red sprout taro,and a total of 773 loci were obtained,including 582 polymorphic loci,with a polymorphic percentage of 74.62%.The total number of loci amplified by different primers varied,with a range of 22～65 polymorphic loci.The range of observed allele numbers(Na)detected by different primers was 1.5946～1.8475,the range of effective allele numbers(Ne)was 1.3117～1.4646,the range of Nei's gene diversity index(H)was 0.1886～0.2707,and the range of Shannon's information index(I)was 0.2882～0.4109.The genetic similarity coefficients of the 9 samples of Jiangxi Yanshan red sprout taro were 0.5964～0.8797,all of which exceed 0.5,indicating that the genetic differences among the 9 samples of Jiangxi Yanshan red sprout taro were relatively small.The clustering analysis results show that shoot-tip conventional culture virus-free,shoot-tip virus-free by encapsulation dehydration cryotherapy,shoot-tip virus-free by encapsulation vitrification cryotherapy,and shoot-tip virus-free by droplet vitrification cryotherapy can not cause genetic variation in Jiangxi Yanshan red sprout taro.However,callus redifferentiation,normal temperature in vitro hormone subculture,and shoot-tip virus-free by vitrification cryotherapy may cause genetic variation in Jiangxi Yanshan red sprout taro.关键词
江西铅山红芽芋/脱毒苗/遗传稳定性/SRAP检测Key words
Jiangxi Yanshan red sprout taro/Virus-free plantlet/Genetic stability/SRAP detection分类
农业科技引用本文复制引用
ZHANG Yi-xin,DU Jing-yi,HE Ling-ting,Adile Wumar,Harimillan Ahemaiti,ZHANG Zhi-yao,MIN Ling-rui,ZHA Shu-li..江西铅山红芽芋脱毒苗遗传稳定性的SRAP检测[J].江西农业学报,2025,37(12):69-78,10.基金项目
国家自然科学基金项目(31860084、32060092). （31860084、32060092）
