日用化学品科学2025，Vol.48Issue(12)：24-30,7.DOI:10.3969/j.issn.1006-7264.2025.12.004
半乳糖酵母发酵滤液抗氧化活性及代谢组学研究
Antioxidant activity and metabolomic analysis of galactomyces yeast fermentation filtrates
XIE Gaobao 1YANG Jiafu 2YE Tongmei 1WANG Tao 1CHEN Laicheng 1SHEN Guofeng1
作者信息
- 1. Guangzhou Mashanghui Biotechnology Co.,Ltd.,Guangzhou,Guangdong 510800,China
- 2. Guangzhou Jucheng Chemical Co.,Ltd.,Guangzhou,Guangdong 510800,China
- 折叠
摘要
Abstract
Three strains of galactomyces yeast were investigated to assess dynamic changes in antioxidant activity during fermentation.The DPPH radical scavenging activity of the fermentation filtrate from strain 2A9 peaked at 75.68%±1.71%(equivalent to 142.5 mg Vc/L)at 87 h,while strains 61B1 and 1H1 reached optimal activity at the fermentation endpoint(96 h).Non-targeted LC-MS metabolomics combined with OPLS-DA modeling identified 30 significantly differentially expressed metabolites(VIP>1,P<0.05),predominantly enriched in the"glutathione metabolism,""pantothenate and CoA biosynthesis,"and"tryptophan metabolism"pathways.Key differential metabolites—including L-glutamic acid,glutathione,hydroxycinnamic acid,acetyl-L-carnitine,L-phenylalanine,and isoflavonoids(genistein,daidzein aglycone)—likely underlie the differences in antioxidant capacity among the three fermentation filtrates.This study elucidates the metabolic profile variations of galactomyces fermentation filtrates,providing a theoretical foundation for their potential applications.关键词
半乳糖酵母/发酵滤液/抗氧化/非靶向代谢组学/差异代谢物Key words
galactomyces yeast/fermentation filtrate/antioxidant activity/non-targeted metabolomics/differential metabolites分类
化学化工引用本文复制引用
XIE Gaobao,YANG Jiafu,YE Tongmei,WANG Tao,CHEN Laicheng,SHEN Guofeng..半乳糖酵母发酵滤液抗氧化活性及代谢组学研究[J].日用化学品科学,2025,48(12):24-30,7.
