肿瘤预防与治疗2025，Vol.38Issue(12)：1033-1041,9.DOI:10.3969/j.issn.1674-0904.2025.12.001
前脂肪细胞通过分泌IL-6促进乳腺癌细胞的增殖、迁移并抑制其凋亡
IL-6 Secretion from Preadipocytes Stimulates Breast Cancer Cell Prolifer-ation,Migration,and Inhibits Apoptosis
摘要
Abstract
Objective:To investigate the effect of preadipocytes on breast cancer cells within the tumor microenvironment and to identify the key cytokines therein.Methods:Co-culture systems of preadipocytes and breast cancer cells were estab-lished using the Transwell chamber method and the conditioned medium method.The Transwell system enables indirect com-munication between cell types through secreted molecules without physical contact.In the conditioned medium model,the se-creted factors from preadipocytes exerted a unidirectional effect on the breast cancer cells.After co-culture,breast cancer cell phenotypes were evaluated by multiple assays,including colony formation,CCK-8,wound healing,JC-1 mitochondrial membrane potential detection,and Hoechst-PI staining.Interleukin-6(IL-6)levels in the conditioned media from different culture conditions were measured by ELISA.Results:Co-culture with preadipocytes could promote the proliferation,viability,and migration of breast cancer cells while suppress-ing their apoptosis.The observed differences were statistically significant.Moreover,the promotional and inhibitory effects correlated positively with the number of preadipocytes and the concentration of IL-6 they secreted.Meanwhile,the phenotyp-ic effects on breast cancer cells were greater with the conditioned medium method than with the Transwell system,and this correlated with higher concentrations of secreted IL-6(P<0.05).Conclusion:Preadipocytes promote breast cancer cell prolif-eration,migration,and viability while suppressing apoptosis,primarily via increased secretion of IL-6.This process is not uni-directionally acted upon by preadipocytes on breast cancer cells;it is also subject to feedback and regulation from tumor cells.关键词
前脂肪细胞/乳腺癌细胞/IL-6/共培养/增殖/迁移/凋亡Key words
Preadipocytes/Breast cancer cells/IL-6/Co-culture/Proliferation/Migration/Apoptosis分类
生物科学引用本文复制引用
Yuan Kedi,Li Shuting..前脂肪细胞通过分泌IL-6促进乳腺癌细胞的增殖、迁移并抑制其凋亡[J].肿瘤预防与治疗,2025,38(12):1033-1041,9.基金项目
This study was supported by grants from Yunnan Development and Reform Commission(No.YNQR-QNRC-2020-103). "兴滇英才支持计划"项目(编号:YNQR-QNRC-2020-103) （No.YNQR-QNRC-2020-103）
