上海交通大学学报（医学版）2025，Vol.45Issue(11)：1458-1465,8.DOI:10.3969/j.issn.1674-8115.2025.11.005
A型有髓纤维DRG神经元内Cav3.2通道N-糖基化介导糖尿病触诱发痛的研究
N-glycosylation of Cav3.2 channel in DRG neurons of myelinated A-fiber contributes to diabetic mechanical allodynia
摘要
Abstract
Objective·To investigate the association of Cav3.2 channel N-glycosylation in myelinated A-fiber dorsal root ganglion(DRG)neurons with diabetic mechanical allodynia(MA).Methods·Diabetic rat models were induced by a single intraperitoneal injection of streptozotocin.Pain behavior assessments,including evaluations of MA and thermal hyperalgesia(TH),were conducted on a weekly basis.Diabetic rats exhibiting TH were subsequently categorized into two subcategories based on the onset of mechanical allodynia by the third week.The impact of N-glycosylation on both the expression of Cav3.2 channels in DRG neurons and pain behaviors was investigated through sequential administration of neuraminidase(NEU)and TTA-P2,a selective blocker of T-type calcium channels,after C-unmyelinated fiber blockage by the application of resiniferatoxin(RTX).The extent of Cav3.2 N-glycosylation was quantitatively compared between diabetic rats with and without MA by analyzing the N-terminal fragment of Cav3.2 following de-glycosylation.Results·Higher levels of Cav3.2 expression in myelinated A-fiber DRG neurons were observed in diabetic rats with MA compared to those without MA(P＜0.001).NEU-induced de-glycosylation led to a reduction in Cav3.2 expression across all groups,with diabetic rats with MA maintaining higher levels of the Cav3.2 expression than those without MA(P＜0.05).It was observed that RTX eliminated only TH and not MA,whereas NEU had a restorative effect on both TH and MA,similar to TTA-P2.Diabetic rats with MA exhibited increased expression of N-terminal fragments compared to those without MA(P＜0.001).Conclusion·N-glycosylation of the Cav3.2 channel within DRG neurons of myelinated A-fiber is a contributing factor to diabetic MA.关键词
糖尿病周围神经病变/Cav3.2通道/触诱发痛/背根神经节/糖基化Key words
diabetic peripheral neuropathy/Cav3.2 channel/mechanical allodynia/dorsal root ganglion/glycosylation分类
医药卫生引用本文复制引用
LIAO Chenlong,LI Shuo,XU Xin,ZHANG Yue,ZHANG Wenchuan..A型有髓纤维DRG神经元内Cav3.2通道N-糖基化介导糖尿病触诱发痛的研究[J].上海交通大学学报（医学版）,2025,45(11):1458-1465,8.基金项目
国家自然科学基金(81801219,82271389) （81801219,82271389）
上海市"科技创新行动计划"医学创新研究专项(21Y11906300) （21Y11906300）
上海市自然科学基金(21ZR1438100). National Natural Science Foundation of China(81801219,82271389) （21ZR1438100）
Medical Innovation Research Project of Science and Technology Commission of Shanghai Municipality(21Y11906300) （21Y11906300）
Natural Science Foundation of Shanghai(21ZR1438100). （21ZR1438100）
