生物信息学2025，Vol.23Issue(4)：304-312,9.DOI:10.12113/202404002
生信分析胃癌中MAPK相关基因表达及IL1A的免疫意义
Bioinformatics analysis of MAPK related gene expression and immune significance of IL1A in gastric cancer
摘要
Abstract
To search for biomarkers related to gastric cancer in MAPK related genes.Differential expression of MAPK-related genes in gastric cancer is analyzed by TCGA,MSigDB database,R-packet limma and wilcoxon test.Survival analysis is performed through R packet survival.Construct PPI network through STRING database.Enrichment analysis is performed by Metascape.The infiltration level of gastric cancer immune cells is calculated by cibersort.Finally,real-time fluorescence quantitative PCR(RT-qPCR)is used to verify the expression of differential genes in 20 gastric cancer and adjacent normal tissue samples.Among the MAPK related genes in gastric cancer,27 are upregulated and 59 are downregulated.Survival analysis revealed that MAPK related genes IL1A,MAP4K4,and PDGFRB are highly expressed in gastric cancer tissue and affect the prognosis of gastric cancer.The PPI network showed that differentially expressed gene proteins have extensive interactions.GO and KEGG functional analysis show that the IL1A,MAP4K4,and PDGFRB genes are closely related to EGFR tyrosine kinase inhibitor resistance,MAPK signaling pathway,and other pathways.Analysis of immune cell infiltration in gastric cancer revealed a significant correlation between IL1A and mast cell infiltration.In RT qPCR validation,the expression of IL1A,MAP4K4,and PDGFRB in gastric cancer tissue(1.90±0.34),(2.10±0.44),(2.08±0.40)was higher than in normal tissue(0.90±0.16),(0.96±0.18),(1.08±0.20).MAPK related genes IL1A,MAP4K4,and PDGFRB are differentially expressed in gastric cancer and are associated with prognosis.Among them,IL1A may serve as a potential biomarker for immunotherapy.关键词
胃癌/MAPK相关基因/生物信息学分析/IL1A/生物标志物Key words
Gastric cancer/MAPK related genes/Bioinformatics analysis/IL1A/Biomarkers分类
生物科学引用本文复制引用
YANG Zhiran,LIU Wenbo,YUAN Xinyu,ZHANG Zhuoqi,HUO Bingjie,GENG Jianlei,LI Yong,TAN Bibo..生信分析胃癌中MAPK相关基因表达及IL1A的免疫意义[J].生物信息学,2025,23(4):304-312,9.基金项目
河北省科技厅重点研发计划(No.22377701D) （No.22377701D）
河北省科技厅2023年度引进国外智力项目(No.7002011) （No.7002011）
河北医科大学"十四五"临床医学创新研究团队支持计划(No.2022LCTD-A13) （No.2022LCTD-A13）
河北省2022年政府资助省级医学优秀人才项目(No.冀财预复[2022]180号) （No.冀财预复[2022]180号）
河北省卫生健康委科研基金项目重点科技研究计划(No.20190756) （No.20190756）
2022年度河北省医学适用技术跟踪项目(No.GZ2022044). （No.GZ2022044）
