中国土壤与肥料Issue(11)：16-29,14.DOI:10.11838/sfsc.1673-6257.25113
砒砂岩区不同年限沙棘人工林根际与非根际土壤细菌群落结构的多样性特征
Diversity characteristics of bacterial community structures in rhizosphere and non-rhizosphere soils of Hippophae rhamnoides plantations with different ages in the arsenic sandstone area
摘要
Abstract
To explore the impact of creating artificial seabuckthorn forests on the soil ecological environment in the arsenic sandstone area of Inner Mongolia,artificial seabuckthorn forests with tree ages of 4 and 9 years were used as research objects,and samples without artificial seabuckthorn forests were used as controls.High-throughput sequencing technology was used to analyze the characteristics of soil bacterial communities,and correlation analysis was conducted to explore the relationships among soil physicochemical properties,enzyme activities,and bacterial community diversity.The results showed as follows:(1)With the increase in planting years,the contents of total potassium(TK),available phosphorus(OP),and pH in the rhizosphere soil of Hippophae rhamnoides forests were all increased,with significant changes in pH(P<0.05).In non-rhizosphere soil,the pH,total phosphorus(TP),available phosphorus(OP),and organic matter(OM)also showed an increasing trend,with significant changes in pH and OM contents(P<0.05).Compared with the control group,the contents of total nitrogen(TN),alkali-hydrolyzable nitrogen(AN),OP,available potassium(AK),and OM in the soil of 4-year-old and 9-year-old Hippophae rhamnoides forests showed significant differences(P<0.05).(2)With the increase in planting years,the activities of three enzymes(urease,invertase and catalase)in rhizosphere soil were all increased,with significant growth in urease and invertase(P<0.05).In non-rhizosphere soil,the activities of invertase and dehydrogenase increased with planting years,while the activities of urease and catalase decreased with planting years,among which the changes in urease and invertase were significant(P<0.05).Compared with the control group,the activities of invertase,catalase,and dehydrogenase in rhizosphere soil were significantly enhanced;the activity of urease in non-rhizosphere soil was significantly reduced(P<0.05).(3)The main dominant phyla of soil bacterial communities in the five treatments included Actinobacteriota,Proteobacteria,Chloroflexi and Acidobacteriota.With the increase in planting years,the relative abundances of Actinobacteriota,Acidobacteriota,and Chloroflexi in rhizosphere soil were increased,while the relative abundance of Proteobacteria was decreased.In non-rhizosphere soil,the relative abundances of Actinobacteriota and Proteobacteria were decreased,while the relative abundances of Chloroflexi and Acidobacteriota were increased.Compared with the control group,significant changes occurred in the community structure and the relative abundances of dominant phyla and genera in the soil samples with artificial Hippophae rhamnoides forests.(4)Redundancy analysis showed that pH was the main factor affecting the distribution of soil bacterial communities and had a positive impact on soil bacterial diversity.In conclusion,establishing artificial Hippophae rhamnoides forests was conducive to improving soil fertility in the arsenic sandstone area of Inner Mongolia and affecting the composition and structure of soil bacterial communities in this area.This study provided an effective supplement for scientifically evaluating the ecological restoration technology of planting Hippophae rhamnoides forests in the arsenic sandstone area for micro-ecological environment regulation.关键词
沙棘/土壤细菌/群落结构/土壤理化性质/土壤酶活性Key words
Hippophile rhomboids L./soil bacteria/community structure/soil physicochemical properties/soil enzyme activity引用本文复制引用
QIAO Zi-wei,JIA Chen-bo,SUN Mei-mei,TIAN Li,ZHANG Xue-ya,GAO Ze-wen..砒砂岩区不同年限沙棘人工林根际与非根际土壤细菌群落结构的多样性特征[J].中国土壤与肥料,2025,(11):16-29,14.基金项目
陕西省重点研发计划(2023-YBNY-250) （2023-YBNY-250）
榆林学院博士科研启动基金(20GK19) （20GK19）
榆林学院研究生创新基金项目(2023YLYCX20). （2023YLYCX20）
