中国土壤与肥料Issue(11)：30-39,10.DOI:10.11838/sfsc.1673-6257.25121
高寒区豆禾牧草单播与混播对根际土壤微生物多样性及养分特性的影响
Effects of single and mixed sowing of soybean and grass on microbial diversity and nutrient characteristics of rhizosphere soil in alpine region
摘要
Abstract
Considering issues such as the cyclical discrepancies in forage availability and the detrimental effects of overgrazing,establishing high-yield,high-quality cultivated grasslands was fundamental for providing essential agricultural products and facilitating the sustainable advancement of animal husbandry.This study investigated the alterations in the composition and diversity of the rhizospheric microbial communities of leguminous forage crops under monoculture and mixed-cropping systems,to provide strategies for improving soil health.High-throughput sequencing technology was employed to analyze Avena sativa L.in a high-altitude cold region.The rhizosphere soil's nutrient profiles and microbial diversity were assessed using unicast and mixed sowing methods.The findings revealed significant variations in available nitrogen,available phosphorus,available potassium,and organic matter levels in the rhizosphere soil samples of oat and arrowpea under monoculture and mixed sowing(P<0.05).The bacterial microbial diversity in the rhizosphere soil was the highest under the oat monoculture system.In contrast,the fungal microbial diversity peaked under the arrowpea monoculture system,with significant differences in rhizosphere microbial diversity observed across various cultivation methods(P<0.05).The predominant bacterial phyla included Proteobacteria(33.66%-37.66%),Acidobacteria(21.96%-26.26%),and Actinobacteria(7.61%-16.77%).The dominant bacterial genera,Sphingomonas and RB41,exhibited significant correlations with total nitrogen,available nitrogen,and organic matter in the soil(P<0.01).The composition of fungal communities across different treatments displayed considerable variation at both the phylum and genus levels,primarily consisting of Ascomycetes and Mortierella.The functional composition of the fungal community was found to be more diverse than that of the bacteria,with substantial differences observed between treatments.The microbial diversity within rhizosphere soil was influenced by various seeding techniques,with notable alterations in community diversity being significantly correlated with total nitrogen,available nitrogen,and organic matter content in the soil.In summary,mixed sowing of legume and grass pastures exhibited favorable soil nutrient status and microbial community structure.This approach offered a solution for addressing imbalances in forage supply in alpine regions through a synergistic"nutrient-microbe-production"mechanism.关键词
高寒地区/燕麦/箭筈豌豆/微生物多样性/播种方式Key words
alpine region/Avena sativa/Vicia sativa/microbial ecosystem diversity/seeding technique引用本文复制引用
DAO Ri-na,ZHANG Ying,WANG Wei,DE Ke-jia,WANG Yan,SU Bei-bei..高寒区豆禾牧草单播与混播对根际土壤微生物多样性及养分特性的影响[J].中国土壤与肥料,2025,(11):30-39,10.基金项目
青海大学科研实力提升项目(2025KTST06). （2025KTST06）
