中国土壤与肥料Issue(11)：40-49,10.DOI:10.11838/sfsc.1673-6257.25125
黄土高原紫花苜蓿土壤原生动物群落组装机制对磷素添加的响应
Response of soil protozoan community assembly to phosphorus addition in alfalfa fields of the Loess Plateau
摘要
Abstract
In order to reveal the effects of phosphorus supplementation on the community function and diversity of soil protozoa of alfalfa,a field experiment was conducted in the semi-arid area of the Loess Plateau,and alfalfa fields with different phosphorus dosages(0,60,120,180 kg/hm2)were used as the research objects.Illumina MiSeq sequencing technology was used to analyze the community characteristics of soil protozoa.The results showed that,compared with CK,P180 reduced Ciliophora,Apicomplexa and Endomyxa by 42.6%,40.7%and 40.8%,respectively.The values of Bacillariophyta,Cercozoa and Oomycota were increased by 36.0%,26.8%and 38.8%,respectively.In addition,there were 2 Photosynthetic protozoa,3 Parasites protozoa,and 6 Consumptive protozoa,with the highest proportion of Consumptive protozoa(72.5%-84.0%).It was followed by parasitic protozoa(12.2%-25.3%)and phototrophic protozoa(2.2%-4.4%).Soil organic carbon,water content and available phosphorus were the main driving factors for the formation of Photosynthetic,Parasites and Consumptive protozoa,respectively.Zero model analysis showed that the assembly of soil protozoa community was dominated by random process(unclear process)at low P level and deterministic process(heterogeneous selection)at high P level.Molecular ecological network analysis showed that there was a strong cooperative relationship between species of soil protozoa community at low P level,while there was a strong competitive relationship between species of soil protozoa community at high P level.The formation of a stable soil environment by alfalfa increased its random process,which was conducive to maintaining the sustainability and stability of artificial grassland ecosystem function.关键词
黄土高原/紫花苜蓿/土壤原生动物/群落组装/共现网络Key words
Loess Plateau/alfalfa/soil protozoa/community assembly/collinear network引用本文复制引用
LI Liang-liang,LUO Zhu-zhu,NIAN Li-li,HE Ren-yuan,LIU Jia-he,JIANG Hai-yang..黄土高原紫花苜蓿土壤原生动物群落组装机制对磷素添加的响应[J].中国土壤与肥料,2025,(11):40-49,10.基金项目
国家自然科学基金项目(31860364、32560778) （31860364、32560778）
甘肃省科技计划项目(24JRRA679) （24JRRA679）
甘肃省优秀研究生创新之星项目(2025CXZX-844、2025CXZX-829). （2025CXZX-844、2025CXZX-829）
