西北农业学报2025，Vol.34Issue(12)：2175-2183,9.DOI:10.7606/j.issn.1004-1389.2025.12.001
CDH11基因过表达载体构建及其对牛成肌细胞增殖和分化的作用
Construction of CDH11 Gene Overexpression Vector and Its Effect on Proliferation and Differentiation of Bovine Myoblasts
摘要
Abstract
The role of cadherin-11(CDH11)gene in the proliferation and differentiation of bovine adult myoblasts was investigated by overexpressing CDH11 and using EdU,CCK-8,immunofluores-cence and quantitative real-time PCR(qRT-PCR).The results showed that CDH11 overexpression significantly increased the EdU positivity and absorbance at 450 nm in bovine myogenic cells(P＜0.05).The expression levels of cyclin-dependent kinase(CDK2)and Proliferating Cell Nuclear Anti-gen(PCNA)were significantly upregulated(P＜0.05).During the myoblast differentiation stage,CDH11 overexpression significantly reduced the area of myotubes and markedly inhibited the myogen-ic differentiation-related genes myogenic differentiation(MYOD),myogenin(MYOG)and myogenic regulatory factors5(MYF5)expression(P＜0.05).These findings indicate that CDH11 overexpres-sion promotes the proliferation of bovine adult myoblasts but inhibits the differentiation of bovine adult myoblasts.关键词
牛成肌细胞/钙粘蛋白11(CDH11)/增殖/分化Key words
Bovine myogenic cells/Calcineurin 11(CDH11)/Proliferation/Differentiation引用本文复制引用
LIU Zhanxin,ZHANG Xiaolan,SHI Bingang,HU Jiang,ZHAO Zhidong,YING Chengyu,BAI Yanbin,NIU Yanmei,JIA Xue,LI Liang,CAI Siyi,WANG Yicheng,ZHANG Junxia,LIU Xuhui..CDH11基因过表达载体构建及其对牛成肌细胞增殖和分化的作用[J].西北农业学报,2025,34(12):2175-2183,9.基金项目
甘肃省青年博士支持项目(2025QB-042) （2025QB-042）
乡村振兴专项(25CXNA058) （25CXNA058）
甘肃农业大学学科团队项目(GAU-XKTD-2022-22). Support Project for Young Ph.D Holders of Gansu Province(No.2025QB-042) （GAU-XKTD-2022-22）
Special Project for Rural Revitalization(No.25CXNA058) （No.25CXNA058）
Discipline Team Project of Gansu Agricul-tural University(No.GAU-XKTD-2022-22). （No.GAU-XKTD-2022-22）
