西北农业学报2025，Vol.34Issue(12)：2202-2213,12.DOI:10.7606/j.issn.1004-1389.2025.12.004
31份哈萨克斯坦小麦种质品质综合评价及其醇溶蛋白组成
Identification and Analysis of Gliadin and Quality Traits in Kazakh Wheat Germplasm Resources
摘要
Abstract
To clarify the comprehensive quality of 31 Kazakh wheat germplasm accessions and investi-gate the influence of gliadin composition on quality traits,parameters such as crude protein content,wet gluten content,water absorption rate,farinograph parameters,gelatinization properties,and the contents of major mineral elements were determined and analyzed.The gliadin composition was ana-lyzed using acid polyacrylamide gel electrophoresis(A-PAGE)technique.The results indicated a wide diversity in gelatinization and farinograph characteristics among the 31 Kazakh wheat germplasm ac-cessions.Based on the comprehensive quality results,eight strong gluten and eight medium-strong glu-ten wheat accessions were identified.Among them,three germplasm met the recommended standards for Ca and Fe contents.A total of 426 gliadin bands were detected,ranging from 6 to 25 per sample,with an average of 13.74 bands per sample.The gliadin band with a migration rate of 69.5 exhibited the highest frequency.The average genetic similarity coefficient(GS)was 0.292,with 92.47％of GS values falling within the range of 0.100 to 0.500.Bands with migration rates of 43.3,51.9,78.0,and 80.7 had highly significant positive effects on water absorption rate,ash content,flour whiteness,and damaged starch,but extremely significant negative effects on minimum viscosity,final viscosity,set-back value,and gluten index.Bands with migration rates of 65.6 and 77.9 had extremely significant positive effects on stability time,while the band at 61.1 showed a highly significant positive effect on wet gluten content(P＜0.01).This study identified strong and medium-strong gluten wheat germ-plasm lines and discovered several gliadin bands significantly correlated with quality traits,providing both germplasm resources and a theoretical basis for wheat quality breeding.关键词
哈萨克斯坦小麦/综合品质/醇溶蛋白Key words
Kazakhstan wheat/Gliadin/Quality trait引用本文复制引用
YU Yinghan,TIAN Hanzhao,YING Kai,SHANG Xuge,ZHANG Zhengmao,Oleg Solovyev,WU Guiling,LIU Baolong,LIU Yuxiu..31份哈萨克斯坦小麦种质品质综合评价及其醇溶蛋白组成[J].西北农业学报,2025,34(12):2202-2213,12.基金项目
国家自然科学青年基金(32101698) （32101698）
陕西省重点研发计划项目(2024GH-ZDXM-07) （2024GH-ZDXM-07）
国家外专项目(DL2022172010L) （DL2022172010L）
优质旱地小麦新品种选育及'普冰322'的示范推广(TGZX2021-9) （TGZX2021-9）
中国科学院"全球共性挑战专项"(077GJHZ2023028GC). National Natural Science Foundation of China(No.32101698) （077GJHZ2023028GC）
Key Research and Development Program of Shaanxi Province(No.2024GH-ZDXM-07) （No.2024GH-ZDXM-07）
National Foreign Expert Project(No.DL2022172010L) （No.DL2022172010L）
Breeding of New High-Quality Dryland Wheat Varieties and Demonstration and Promotion Project of'Pu Bing 322'(No.TGZX2021-9) （No.TGZX2021-9）
"Special Program on Global Common Challenges"of Chinese Academy of Sciences(No.077GJHZ2023028GC). （No.077GJHZ2023028GC）
