西北农业学报2025，Vol.34Issue(12)：2214-2221,8.DOI:10.7606/j.issn.1004-1389.2025.12.005
马铃薯StCYP83B1基因RNAi遗传转化及功能分析
Generationand Functional Analysis of Potato StCYP83B1 RNAi Transgenic Lines
摘要
Abstract
Cytochrome P450(CYP450)is aheme-containing enzyme belonging to the B-family of the cytochrome P450 superfamily that plays a critical role in plant responses to biotic or abiotic stresses.Previous studies have identified a CYP450 gene,StCYP83B1 in potato,which is significantly induced upon infection by Phytophthora infestans;its overexpression enhances pattern-triggered immunity(PTI)and resistance to P.infestans without a growth penalty in potatoes.To further elucidate the role of StCYP83B1 in coordinating potato immunity and growth,this study generated StCYP83B1-RNAi transgenic potatoes via Agrobacterium-mediated genetic transformation.Comparative analyses with control plants('Atlantic')in immune response validation and growth parameter measurements demonstrated that StCYP83B1-RNAi plants exhibited reduced resistance to P.infestans,significantly down-regulated expression of PTI marker genes,and attenuated the flg22-induced reactive oxygen species(ROS)burst.Additionally,the StCYP83B1-RNAi plants showed significant decreases in plant height,leaf number,and stem diameter.These findings preliminarily confirm that StCYP83B1 posi-tively regulates disease resistance and is essential for maintaining normal growth in potato.This study not only provides a theoretical reference for molecular resistance breeding,but also provides critical material for further mechanistic investigation of how StCYP83B1 coordinates potato immunity and growth.关键词
马铃薯/StCYP83B1/致病疫霉/抗病性/生长Key words
Potato/StCYP83B1/Phytophthora infestans/Disease resistance/Growth引用本文复制引用
ZONG Deqian,KONG Lehui,JIAO Wenjing,WANG Bianbian,GUO Shuhan,SHAN Weixing,QIANG Xiaoyu..马铃薯StCYP83B1基因RNAi遗传转化及功能分析[J].西北农业学报,2025,34(12):2214-2221,8.基金项目
陕西省重点研发计划"马铃薯种业创新工程项目"(2021LLRH-07) （2021LLRH-07）
国家自然科学基金(31872657) （31872657）
国家重点研发计划子课题(2017YFD0200602-2). Shaanxi Provincial Key R&D Program"Potato Seed Industry Innovation Project"(No.2021LLRH-07) （2017YFD0200602-2）
National Natural Science Foundation of China(No.31872657) （No.31872657）
Sub-project of the National Key R&D Program of China(No.2017YFD0200602-2). （No.2017YFD0200602-2）
