西北农业学报2025，Vol.34Issue(12)：2231-2242,12.DOI:10.7606/j.issn.1004-1389.2025.12.007
舞草属叶绿体基因组特征及系统发育分析
Comparison of Chloroplast Genomic Characteristics and Phylogenetic Analysis of Codariocalyx Hassk
摘要
Abstract
Codariocalyx Hassk.contains two species,C.motorius and C.gyroides.The lateral leaf-lets of C.motorius can swing up and down nearly 180° or rotate 360°.To investigate the chloroplast genome characteristics and phylogenetic relationships of the genus Codariocalyx,this study was based on high-throughput sequencing using the Illumina platform.Bioinformatics tools such as GetOr-ganelle and CPStools were employed to assemble and analyze the chloroplast genomes.This study fo-cused on their structural features,repeat sequences,sequence divergence,codon usage bias,and phylo-genetic placement within subtribe Desmodiinae.The results showed:(1)The total lengths of the chloroplast genomes of C.motorius and C.gyroides were 149 381 bp and 149 358 bp,respectively,with a total GC content of 35％.A total of 128 genes were identified,including 83 protein-coding genes.(2)SSR sequences in C.motorius were predominantly composed of T and AT repeats,where-as in C.gyroides,A and AT repeats dominated.Notably,only C.gyroides contained a pentanucleoti-de repeat(TTATA).Dispersed repetitive sequences in both species mainly consisted of palindromic repeats and direct repeats.(3)Sequence divergence analysis identified four highly variable regions(trnP-UGG_psaJ,psbD_trnT-GGU,trnY-GUA_trnD-GUC and psaI_ycf 4).(4)C.motorius and C.gyroides formed a single branch,belonging to the Desmodium group of the tribe Desmodieae,and showed the closest phylogenetic relationship with Grona.This study provides a theoretical basis for species identification,genetic diversity research,and resource development and utilization of C.moto-rius and C.gyroides.关键词
舞草属/叶绿体基因组/结构特征/密码子偏好性/系统发育Key words
Codariocalyx/Chloroplast genome/Structural characteristics/Codon usage bias/Phy-logeny引用本文复制引用
HE Aoxiang,ZHOU Shengmao,WANG Fang,ZHAO Xueli..舞草属叶绿体基因组特征及系统发育分析[J].西北农业学报,2025,34(12):2231-2242,12.基金项目
云南省"兴滇英才支持计划"青年人才项目(XDYC-QNRC-2023-0228) （XDYC-QNRC-2023-0228）
国家自然科学基金(31800170). Young Elite Scientists for"Yunnan Revitalization Talent Support Program"of Yun-nan Province(No.XDYC-QNRC-2023-0228) （31800170）
National Natural Science Foundation of China(No.31800170). （No.31800170）
