西北农业学报2025，Vol.34Issue(12)：2252-2262,11.DOI:10.7606/j.issn.1004-1389.2025.12.009
铵硝配比对娃娃菜硫苷合成与代谢的影响
Effects of Ammonium-to-Nitrate Ratio on Synthesis and Metabolism of Glucosinolate in Mini Chinese Cabbage
摘要
Abstract
To clarify the effects of different ammonium-to-nitrate ratios on the synthesis and metabo-lism of glucosinolates in mini Chinese cabbage,and to provide guidance for nitrogen fertilization man-agement to cultivate mini Chinese cabbage with high glucosinolate content,a hydroponic experiment was conducted.Five ammonium-to-nitrate ratios(0∶10,3∶7,5∶5,7∶3 and 10∶0),with a total ni-trogen concentration of 5 mmol/L,were tested.The total amino acid content was highest at the am-monium-to-nitrate ratio of 7∶3.The content of precursor amino acids for glucosinolate synthesis also increased with the higher ammonium nitrogen concentration,thereby promoting glucosinolate synthe-sis.Compared with the total nitrate treatment,the total ammonium treatment(10∶0)upregulated the relative expression levels of BrCYP83A1 and BrCYP83B1 genes,thereby enhancing glucosinolate synthesis.In contrast,compared with the total ammonium treatment,the relative expression of BrESM gene was downregulated in the 7∶3 ammonium-to-nitrate treatment,reducing glucosinolate degradation and increasing glucosinolate accumulation.Thus,increasing ammonium nitrogen content in the nutrient solution can promote glucosinolates synthesis,reduce its degradation,and enhance its accumulation in mini Chinese cabbage.关键词
铵硝配比/娃娃菜/硫苷/基因表达量Key words
Ammonium nitrate ratio/Mini Chinese cabbage/Glucosinolates/Gene expression level引用本文复制引用
GUO Jinze,MAO Lili,YANG Jiaojiao,WANG Xuehua,YU Jihua,HU Linli..铵硝配比对娃娃菜硫苷合成与代谢的影响[J].西北农业学报,2025,34(12):2252-2262,11.基金项目
干旱生境国家重点实验室主任基金(GSCS-2023-Z06) （GSCS-2023-Z06）
甘肃省自然科学基金(21JR7RA803) （21JR7RA803）
甘肃农业大学科技创新基金青年导师扶持基金(GAU-QDFC-2022-12). Director Fund of the State Key Laboratory of Aridland Crop Science(No.GSCS-2023-Z06) （GAU-QDFC-2022-12）
Key Project of the Natural Science Foundation of Gansu Province(No.21JR7RA803) （No.21JR7RA803）
Fund for Youth Mentors of Gansu Agricultural University(No.GAU-QDFC-2022-12). （No.GAU-QDFC-2022-12）
