祁连山野生鳞伞的鉴定、生物学特性与驯化栽培
Identification,Biological Characteristics and Domestication of Wild Pholiota Species in Qilian Mountains
摘要
Abstract
Toexploit wild edible mushroom resources in the Qilian Mountains and facilitate their do-mestication,strain Pa-01 was isolated from wild Pholiota fruiting bodies collected in the Gansu Qilian Mountain National Nature Reserve.Strain Pa-01 was identified as Pholiota adiposa(Batsch)P.Kumm.through morphological observation and ITS sequence analysis.The effects of temperature,pH,carbon and nitrogen sources,carbon-to-nitrogen ratio(C/N),inorganic salts,and vitamins on my-celial growth were systematically investigated.Results showed optimal growth at 22 ℃ and pH 6.5,with fructose as the preferred carbon source,yeast extract as the optimal nitrogen source,and a C/N ratio of 20∶1.Supplementation with MnSO4 and vitamin B12(VB12)significantly enhanced mycelial biomass accumulation.Orthogonal experiments revealed the optimal culture conditions(A3B1C3D3)as follows:mannitol(carbon source),yeast extract(nitrogen source),MnSO4 supplementation,and incubation at 26 ℃.Cultivation trials utilizing locally sourced substrates achieved a biological efficien-cy of 110％for fruiting bodies using a mixture of corn cob(83％),wheat bran(15％),and lime(2％).This study provides the first comprehensive analysis of the biological traits and cold-adaptive mechanisms of P.adiposa in the Qilian Mountains.The corn cob-based,cost-effective cultivation protocol offers a sustainable strategy for wild germplasm conservation and sustainable utilization.关键词
祁连山/多脂鳞伞/生物学特性/驯化栽培/玉米芯Key words
Qilian Mountains/Pholiota adiposa/Biological characteristics/Domestication and cultiva-tion/Corn cob引用本文复制引用
NIU Xin,ZHAO Qian,YANG Ruoxuan,JING Changyue,LU Huili,ZHANG Jianning,LIANG Qianqian,KE Shanwen..祁连山野生鳞伞的鉴定、生物学特性与驯化栽培[J].西北农业学报,2025,34(12):2263-2274,12.基金项目
陇原青年创新创业人才项目(2024QNGR21) （2024QNGR21）
甘肃省自然科学基金(23JRRG0031) （23JRRG0031）
甘肃省科技厅重点研发计划(23YFNG0006) （23YFNG0006）
甘肃省重点人才项目(2023RCXM34) （2023RCXM34）
河西学院博士科研启动基金(KYQD2020038) （KYQD2020038）
甘肃省大学生创新训练项目(S202210740104,S202410740048). Gansu Province Youth Innovation and Entrepreneurship Talent Project(No.2024QNGR21) （S202210740104,S202410740048）
Natural Science Foundation of Gansu Province(No.23JRRG0031) （No.23JRRG0031）
Key Research and Development Program of Gansu Provincial Department of Science and Technology(No.23YFNG0006) （No.23YFNG0006）
Key Talent Program of Gansu Province(No.2023RCXM34) （No.2023RCXM34）
Doctoral Research Ini-tiation Fund Project of Hexi University(No.KYQD2020038) （No.KYQD2020038）
Gansu Provincial College Students'Innovation and Entrepreneurship Training Program(No.S202210740104,No.S202410740048). （No.S202210740104,No.S202410740048）
