西南林业大学学报2025,Vol.45Issue(11):38-44,7.DOI:10.11929/j.swfu.202410047
棒叶落地生根胎生苗对NaCl胁迫的耐受性评价
Evaluation of the Tolerance of Kalanchoe delagoensis Viviparous Seedlings to NaCl Stress
摘要
Abstract
In order to investigate the mechanisms reflecting the growth and physiological characteristics of Kalanchoe delagoensis viviparous seedlings under NaCl stress,adventitious buds at the top of K.delagoensis leaves were used as materials,using nutrient bags filled substrate cultivation and saline water irrigation for roots method to control soil salinity,and 5 concentration gradients were set for NaCl,which were 0,0.1,0.2,0.3 and 0.5 mol/L,the changes of growth and physiological indexes of the rooted viviparous seedlings of K.delagoensis were investigated under the stress conditions of different NaCl concentrations,and evaluated by the principal compon-ent analysis.The results showed that the growth and physiological performances of the rooted viviparous seed-lings under NaCl stress showed different degrees of responses,especially with the increase of NaCl stress concen-tration,the plant height and leaf water content decreased,the number of rod leaves increased and then decreased,while the root system elongated,but the difference of the leaf width was not significant compared with that of the control.With the time extension of NaCl stress,the chlorophyll,soluble sugar,malondialdehyde contents,SOD and POD activities were increasing,and soluble protein,starch and proline contents,as well as CAT,PAL and PPO activities were firstly increasing and then decreasing,which showed that the fetal seedling enhanced its NaCl stress tolerance by regulating the contents of osmoregulatory substances and antioxidant enzyme activities in vivo.Based on the growth indicator with a salt tolerance threshold of 50%,seedlings could survive at a NaCl concentra-tion of 0.5 mol/L,but most growth and physiological indicators are inhibited,indicating that the K.delagoensis could adapt to NaCl stress below 0.5 mol/L.关键词
棒叶落地生根/胎生苗/NaCl胁迫/生长指标/生理Key words
Kalanchoe delagoensis/viviparous seedling/NaCl stress/growth index/physiology分类
农业科技引用本文复制引用
Li Cuiping,Ai Xingmei,Wan Min,Chen Miaomiao..棒叶落地生根胎生苗对NaCl胁迫的耐受性评价[J].西南林业大学学报,2025,45(11):38-44,7.基金项目
云南省农业基础研究联合专项面上项目(202301BD070001-119)资助 (202301BD070001-119)
西南林业大学博士科研基金项目(111806)资助. (111806)