临床医学工程2025，Vol.32Issue(12)：1317-1320,4.DOI:10.3969/j.issn.1674-4659.2025.12.1317
p16/Ki-67免疫组织化学染色在子宫颈高级别鳞状上皮内病变和子宫颈鳞癌诊断中的临床价值
Clinical Value of p16/Ki-67 Immunohistochemical Staining in the Diagnosis of High-Grade Squamous Intraepithelial Lesions and Cervical Squamous Cell Carcinoma
摘要
Abstract
Objective To explore the clinical value of p16/Ki-67 immunohistochemical staining in the diagnosis of high-grade squamous intraepithelial lesions(HSIL)and cervical squamous cell carcinoma.Methods 252 specimens diagnosed with cervicitis(cervicitis group),173 specimens of cervical LSIL(LSIL group),97 specimens of cervical HSIL(HSIL group),and 61 specimens of cervical squamous cell carcinoma(cervical squamous cell carcinoma group)were selected as research subjects.The remaining ultra-thin liquid based cytology specimens(TCT)from the four groups of research subjects were retrospectively used to detect p16/Ki-67 immunohistochemical staining in TCT thin sections and tissue sections of the four groups of research subjects.The efficacy of p16/Ki-67 immunohistochemical staining in the diagnosis of HSIL and cervical squamous cell carcinoma was evaluated.Results The overall difference in the positivity rate of p16/Ki67 immunohistochemical staining among different diagnostic levels of cervical cytology was statistically significant(P<0.05).The positivity rate of p16/Ki67 immunohistochemical staining was highest in the ASC-H and above lesion groups,and the difference was statistically significant compared with the NILM group,ASCUS group,and LSIL group(P1=0;P2=0;P3=0).The overall difference in the positivity rate of p16/Ki67 immunohistochemical staining among different diagnostic levels of cervical histology was statistically significant(P<0.05).The positive rate of p16/Ki67 immunohistochemical staining was highest in the cervical squamous cell carcinoma group,and the difference was statistically significant compared with the cervicitis group and LSIL group(P1=0;P2=0).The sensitivity,specificity,conformity rate,Youden index,negative predictive value,and positive predictive value of p16/Ki67 immunohistochemical staining in the diagnosis of HSIL and cervical squamous cell carcinoma were 92.41%,84.24%,86.45%,0.77,96.76%,and 68.54%,respectively.Conclusions The p16/Ki-67 immunohistochemical staining gradually increases with the aggravation of cervical cytology diagnosis results and cervical histology diagnosis results.P16/Ki-67 immunohistochemical staining has a high comprehensive efficacy in the diagnosis of HSIL and cervical squamous cell carcinoma,and has high clinical value for promotion and application.关键词
p16/Ki-67/免疫组织化学/高级别鳞状上皮内病变/低级别鳞状上皮内病变/子宫颈鳞癌Key words
p16/Ki-67/Immunohistochemistry/High-grade squamous intraepithelial lesion/Low-grade squamous intraepithelial lesion/Cervical squamous cell carcinoma分类
医药卫生引用本文复制引用
CAI Chunfang,DENG Wentong,HUANG Huayong..p16/Ki-67免疫组织化学染色在子宫颈高级别鳞状上皮内病变和子宫颈鳞癌诊断中的临床价值[J].临床医学工程,2025,32(12):1317-1320,4.基金项目
广东省中医药管理局项目(项目编号:20201300) （项目编号:20201300）
