云南民族大学学报（自然科学版）2025，Vol.34Issue(6)：631-637,7.DOI:10.3969/j.issn.1672-8513.2025.06.001
川贝母基源植物鳞茎内生真菌产生物碱菌株筛选及活性测定
Screening of alkaloid-producing strains and activity determination of endophytic fungi from the bulbs of Fritillaria cirrhosa plants
摘要
Abstract
Endophytic fungal strains from the bulbs of Fritillaria cirrhosa plants were screened for their ability to metabolize and produce the alkaloid active ingredients of F.cirrhosa,and their antioxidant and antibacterial activi-ties were evaluated.Alkaloid color reaction,thin layer chromatography((TLC),and high-performance liquid chromatography-evaporative light scattering detection(HPLC-ELSD)were used to detect the alkaloid compo-nents in the fermentation products of the endophytic strains.The antioxidant activity of the fermentation products was evaluated by determining the DPPH free radical scavenging capacity,ABTS free radical scavenging capacity,and ferric reducing antioxidant power(FRAP),while the antibacterial activity was evaluated using the agar well diffusion method.It was found that four endophytic fungi strains of Fusarium(Fu.tricinctum,Fu.oxysporum,Fu.solani and Fu.sp.)were able to produce the alkaloid active components of sipeimine,sipeimine-3-β-D-glucoside,and peimisine,which are the same as or similar to those found in F.cirrhosa.The trichlorometh-ane extract of a Fu.petersiae strain exhibited strong antioxidant activity,and the total alkaloid components of a Phanerochaete sp.strain showed good antibacterial activity.In conclusion,endophytic fungi of the genus Fusarium demonstrate potential for producing alkaloid active ingredients characteristic of F.cirrhosa,and their fer-mentation products exhibit notable antioxidant activity,warranting further research and development for practical applications.关键词
川贝母/内生真菌/生物碱/抗氧化活性/抑菌活性Key words
Fritillaria cirrhosa/endophytic fungi/alkaloids/antioxidant activity/antibacterial activity分类
医药卫生引用本文复制引用
XIONG Yu-chun,PENG Hui-zhen,GAO Qian,DONG Fa-wu..川贝母基源植物鳞茎内生真菌产生物碱菌株筛选及活性测定[J].云南民族大学学报（自然科学版）,2025,34(6):631-637,7.基金项目
云南省科学技术厅云南中医学院应用基础研究联合专项资金(2018FF001(-038) （2018FF001(-038）
2017FF116(-005)) （-005）
云南省科技人才和平台计划(202105AG070012). （202105AG070012）
