云南民族大学学报（自然科学版）2025，Vol.34Issue(6)：688-693,6.DOI:10.3969/j.issn.1672-8513.2025.06.007
淫羊藿素通过抑制TGF-β/Smad2信号通路发挥抗肝纤维化作用机制研究
Study on the mechanisms underlying the anti-fibrogenic effects of icaritin in the liver by inhibiting TGF-β/Smad2 signaling pathway
摘要
Abstract
To investigate the effects of icaritin on TGF-β1-induced activation of human/rat hepatic stellate cells(HSC-LX-2/HSC-T6)and the related mechanisms,HSC-LX-2 and HSC-T6 cells were cultured in vitro and divided into the solvent control group,icaritin-alone group,TGF-β1-induced group,and TGF-β1 plus icaritin groups at low,medium,and high concentrations.The protein expression levels of TβRI/II,p-Smad2,t-Smad2,α-SMA,fibronectin,and collagen I were detected by Western blot.The intracellular distribution of p-Smad2 in HSC-LX-2 and HSC-T6 cells was detected by immunofluorescence staining.The results showed that icaritin,at different concentrations,could significantly inhibit the protein expression of TβRI,p-Smad2,α-SMA,fibronectin,and collagen I in TGF-β1-activated hepatic stellate cells in a concentration-dependent manner.Immunofluorescence results showed that icaritin treatment significantly inhibited the TGF-β1-induced nuclear translocation of p-Smad2.Icaritin could prevent the activation of hepatic stellate cells and the synthesis of extracellular matrix components induced by TGF-β1,thereby exerting an anti-hepatic fibrosis effect in vitro,which may be associated with the inhibition of the TGF-β/Smad2 pathway.关键词
淫羊藿素/TGF-β1/肝纤维化/肝星状细胞/Smad2Key words
icaritin/TGF-β1/hepatic fibrosis/hepatic stellate cells/Smad2分类
医药卫生引用本文复制引用
HUANG Zhao,LIU Yu-qi,LI Ao,WANG Wei-guang..淫羊藿素通过抑制TGF-β/Smad2信号通路发挥抗肝纤维化作用机制研究[J].云南民族大学学报（自然科学版）,2025,34(6):688-693,6.基金项目
国家自然科学基金(31960095) （31960095）
云南省应用基础项目(202101AS070022). （202101AS070022）
