云南民族大学学报（自然科学版）2025，Vol.34Issue(6)：694-700,7.DOI:10.3969/j.issn.1672-8513.2025.06.008
姜黄素通过NOX4/ROS/GSDMD轴诱导焦亡抑制HepG2细胞生长
The NOX4/ROS/GSDMD axis in curcumin-induced pyroptosis and growth inhibition of HepG2 cells
摘要
Abstract
The study explores the effects and molecular mechanisms of curcumin(Cur)on pyroptosis in human hepatocellular liver carcinoma cells(HepG2).The effects of curcumin on cell viability,pyroptosis,apoptosis,and oxidative stress in HepG2 cells,along with its regulatory mechanism on pyroptosis,were investigated using CCK-8 assay,Annexin V-FITC/PI double staining,DCFH-DA fluorescent probing,Western blot analysis,and enzyme-linked immunosorbent assay(ELISA).The results showed that the viability of HepG2 cells was significantly inhibited by curcumin in a dose-dependent manner;curcumin-treated HepG2 cells displayed abnormal morphology with many vacuoles,and there was a significant increase in LDH,IL-1β,and IL-18.Intracellular levels of ROS and MDA in HepG2 cells were significantly up-regulated by curcumin in a dose-dependent manner.Furthermore,curcumin notably promoted the phosphorylation of NF-κB in HepG2 cells as well as increased the expression levels of NLRP3,NOX4,caspase-1,and gasdermin D(GSDMD)proteins.The results suggest that curcumin may induce pyroptosis in HepG2 cells by activating the NOX4/ROS/GSDMD axis,promoting NLRP3 inflammasome assembly,which subsequently activates Caspase-1 and cleaves GSDMD,leading to the release of LDH,IL-1β,and IL-18.关键词
姜黄素/NOX4/GSDMD/HepG2细胞/细胞焦亡Key words
curcumin/NOX4/GSDMD/HepG2 cells/pyroptosis分类
医药卫生引用本文复制引用
ZHU Ming-xiu,LI Ping..姜黄素通过NOX4/ROS/GSDMD轴诱导焦亡抑制HepG2细胞生长[J].云南民族大学学报（自然科学版）,2025,34(6):694-700,7.基金项目
全国名老中医传承工作室项目(皖中医药发展秘[2022]19号). （皖中医药发展秘[2022]19号）
