亚热带植物科学2025，Vol.54Issue(5)：487-496,10.DOI:10.3969/j.issn.1009-7791.2025.05.001
多腺拟蝶唇兰质体基因组特征及其系统发育分析
Plastid Genome and Phylogenetic Analysis of Psychopsis sanderae
摘要
Abstract
Using the leaves of the ornamental orchid Psychopsis sanderae as materials,based on high-throughput sequencing technology,the complete plastid genome of this species was assembled and annotated,and its plastid genome characteristics and phylogenetic position were analyzed.The results showed that the plastid genome of P.sanderae was 143 932 bp in size,presenting a circular quadripartite structure,with a GC content of 37.1%.A total of 127 genes were annotated,including 74 protein-coding genes,38 transfer RNAs(tRNAs)genes,8 ribosomal RNAs(rRNAs)genes and 7 pseudo genes.Among them,the entire lineage of the ndh gene family was pseudogenized or lost.The boundaries of the inverted repeat(IR)regions were highly conserved.The junction of the large single copy(LSC)region and the IRb region was located within the rpl22 gene,and the junction of the small single copy(SSC)region and the IRa region was located within the ycf1 gene.A total of 44 simple sequence repeats(SSRs)loci were detected,among which the mononucleotide repeats with adenine(A)or thymine(T)were the most abundant,accounting for 70.45%.Six high-variation hotspots of nucleotide polymorphism were obtained:trnQUUG,trnEUUC,trnSGGA,rps18,rps12,and rpl16.Phylogenetic analyses placed P.sanderae at the basal position within the subtribe Oncidiinae.This study provides the first comprehensive characterization of the plastid genome for P.sanderae,laying a foundation for future molecular marker development and phylogenetic studies of Psychopsis and its relatives.关键词
多腺拟蝶唇兰/质体基因组/分子系统发育/文心兰亚族Key words
Psychopsis sanderae/plastid genome/phylogenetic analysis/Oncidiinae分类
农业科技引用本文复制引用
XU Jing,PAN Ren-fu,HUANG Si-ming,HUANG Wei-chang,LI Ming-he..多腺拟蝶唇兰质体基因组特征及其系统发育分析[J].亚热带植物科学,2025,54(5):487-496,10.基金项目
国家自然科学基金项目(32271957) （32271957）
