亚热带植物科学2025，Vol.54Issue(5)：516-522,7.DOI:10.3969/j.issn.1009-7791.2025.05.004
素馨花多糖提取工艺优化及其抗氧化活性分析
Optimization of Extraction Process and Antioxidant Activity of Polysaccharides from Jasminum grandiflorum
摘要
Abstract
This study aimed to optimize the process of extracting crude polysaccharides from Jasminum grandiflorum by water extraction and explore its antioxidant activity.Taking the polysaccharide yield as the index,based on single-factor experiments,the Box-Behnken(BBD)response surface methodology was adopted to optimize the extraction process.The J.grandiflorum polysaccharides(SP)were obtained by removing proteins and small-molecule impurities through the Sevage method and dialysis,and their antioxidant activities were determined.The results showed that the optimal extraction process conditions for crude polysaccharides from J.grandiflorum were a liquid-to-solid ratio of 15 mL·g-1,an extraction temperature of 94℃,and an extraction time of 2.5 h.Under these process conditions,the average yield of crude polysaccharides from J.grandiflorum was 12.25%,which was close to the predicted extraction rate of 12.24%.The results of antioxidant activity determination showed that SP had scavenging rates of 80.83%and 69.63%for 1,1-diphenyl-2-picrylhydrazyl(DPPH)radicals and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)diammonium salt(ABTS)radicals,respectively.In addition,the IC50 values were 2.04 mg·mL-1 and 3.60 mg·mL-1,respectively,indicating its antioxidant activity.In conclusion,the water extraction-alcohol precipitation method is simple,efficient,and suitable for the extraction of SP,which have antioxidant potential.关键词
素馨花/多糖/工艺优化/抗氧化活性Key words
Jasminum grandiflorum/polysaccharides/process optimization/antioxidant activity分类
农业科技引用本文复制引用
CHEN Cai-hong,HU Gui-e,WANG Wen-feng,LI Dong-li..素馨花多糖提取工艺优化及其抗氧化活性分析[J].亚热带植物科学,2025,54(5):516-522,7.基金项目
广东省科技计划项目-粤澳科技创新联合资助专题(2024A0505090024) （2024A0505090024）
