湖北医药学院学报2025，Vol.44Issue(6)：663-671,9.DOI:10.13819/j.issn.2096-708X.2025.06.003
空间转录组学联合单细胞测序分析E2F5在口腔鳞状细胞癌中的高表达特征
Integrated Analysis of Spatial Transcriptomics and Single-Cell Sequencing Reveals High Expression Characteris-tics of E2F5 in Oral Squamous Cell Carcinoma
摘要
Abstract
Objective To investigate the expression pattern of E2F Transcription Factor 5(E2F5)in oral squamous cell car-cinoma(OSCC),its effect on cell proliferation,and its relationship with the immune microenvironment.Methods Spatial transcriptomic data from the 10X Genomics Visium platform were used to analyze the spatial distribution of E2F5.Single-cell RNA sequencing data were used to analyze the expression patterns of E2F5 across different cell subpopulations.Multi-center data were integrated to assess the expressions of E2F5 using standardized mean difference(SMD)and summary re-ceiver operating characteristic(sROC)curves.The relationship between E2F5 and clinicopathological parameters was also analyzed.CRISPR knockout screening was applied in evaluating,the effect of E2F5 on cell proliferation,and the correla-tion between E2F5 and immune infiltration was evaluated.Results Spatial transcriptomics revealed that E2F5 was expressed at high levels in the tumor epithelial regions of OSCC,and spatially co-localized with markers including KRT5,KRT14,and TP63.Single-cell sequencing analysis of 679,641 cells indicated that E2F5 was predominantly highly expressed in epi-thelial cell subpopulations,particularly in KRT5+/KRT14+/TP63+/MKI67+proliferative epithelial cells.E2F5 mRNA was significantly upregulated in OSCC tissues(SMD=0.28,95%CI=0.06-0.51).High E2F5 expression had a remarkable association with T stage,lymph node metastasis,clinical stage,histological grade,gender,race,and the history of alcohol consumption(P＜0.05).CRISPR knockout screening demonstrated that OSCC cell proliferation was markedly inhibited after E2F5 depletion(SCORE＜0).It was found from immune infiltration analysis that high E2F5 expression was negatively cor-related with multiple immune cell enrichment scores.Conclusion E2F5 promotes the OSCC cell proliferation possibly by regulating the cell cycle and the immune microenvironment.关键词
E2F转录因子5/口腔鳞状细胞癌/空间转录组/单细胞测序/CRISPRKey words
E2F transcription factor 5/Oral squamous cell carcinoma/Spatial transcriptomics/Single-cell sequencing/CRISPR引用本文复制引用
WU Ke-jun,LI Dong-ming,ZHANG Jia-ming,LI Yu-feng,LI Jian-di,MO Hong-bo,WANG Na,CHEN Guo-qiang,CHEN Yu,CHEN Gang..空间转录组学联合单细胞测序分析E2F5在口腔鳞状细胞癌中的高表达特征[J].湖北医药学院学报,2025,44(6):663-671,9.基金项目
广西壮族自治区卫生健康委员会科研项目(Z-A20220459) （Z-A20220459）
